How to translate text using browser tools
1 April 2018 Wright Stain and Fluorescence-Based Methods in Tetrahymena for Phenotypic Analysis
MaríA Del Rosario Ramírez Mata, Robert M. Kao
Author Affiliations +

In educational and research settings, Tetrahymena is an excellent model organismfor engaging students to investigate function, morphology, structure, phagocytosis, and ciliary motion. Here, we present applications of Wright stain and Sytox green as useful low-cost tools for phenotypic analysis. We used heat-fixed Tetrahymena followed by Wright-stain-labeled organelles at different stages of its life cycle. In addition, a low concentration of Wright stain, at 1 percent (vol/vol), enabled visualization of filled vacuoles with stain in live Tetrahymena. Furthermore, we identified that Sytox green fluorescence labels not only nuclei of pre-incubated cultures of Tetrahymena, but also nuclei and some notable cytoplasmic staining after heat fixation. These applications can be used alongside inverted, battery-operated, bright field, fluorescence microscopes (Miller et al., 2010), as well as Cellcams Martin & Shin, 2016) for acquiring images and time-lapse movies. In the future, these useful approaches can be applied broadly in many lab inquiry settings, such as toxicology and molecular genetics.

© 2018 National Association of Biology Teachers. All rights reserved. Please direct all requests for permission to photocopy or reproduce article content through the University of California Press's Reprints and Permissions web page,
MaríA Del Rosario Ramírez Mata and Robert M. Kao "Wright Stain and Fluorescence-Based Methods in Tetrahymena for Phenotypic Analysis," The American Biology Teacher 80(4), 312-317, (1 April 2018).
Published: 1 April 2018

This article is only available to subscribers.
It is not available for individual sale.

heat fixation
live cells
phenotypic analysis
Sytox green dye
Wright stain
Get copyright permission
Back to Top