Pesticides and their associated modes of action serve as real-world examples of chemical toxicity, stimulating student interest and supporting their understanding of nervous system function and cell signaling. An open-source web application called “Neuron-to-Neuron Normal and Toxic Actions” hosts narrated animations of pesticide toxic actions and exists as a resource for instructors of advanced secondary or undergraduate biology courses. This article describes the features of the web application, reports student feedback on the animations, and details a cooperative learning procedure for instructors to use the web application in online learning environments or in-person classroom settings with technology support.