A large emergence of bats from a roost tree or more commonly a cave, provide birds of prey, especially owls, with an improved opportunity to capture bats. Away from these concentrated bat communities, bats are thought to make up an insignificant portion of owl diets. However, little research has investigated if bats perceive owls as a potential predatory threat. We conducted acoustic bat surveys to assess if bat activity was altered by the presence of owl calls or ambient nocturnal noise. Our surveys found no difference in the level of bat activity recorded before or during broadcasted owl calls or nocturnal noise. It is possible bats do not respond to calling owls because bats do not perceive owls as a threat. Additional studies may elucidate the relationship (if any) between bats and owls.