Physical and environmental barriers drive evolutionary diversification by limiting gene flow among populations. Rivers are barriers to gene flow in birds and other vertebrates, but differences in ecological conditions among sites also can affect the genetic structure of populations. The Magdalena River Valley (MRV) of Colombia, the largest South American river valley west of the Andes, is an appropriate location in which to test for the joint role of physical and ecological barriers because the river separates populations to the east and west, and a marked precipitation gradient leads to the occurrence of dry forests in the south and wet forests in the north. We conducted phylogeographic and population genetic analyses using mitochondrial DNA sequences of 4 avian species (Xiphorhynchus susurrans, Mionectes oleagineus, Leptopogon amaurocephalus, and Eucometis penicillata) sampled across 15 localities along the MRV. We found no spatial genetic structure in any of the species in the MRV region, and thus failed to find evidence for the role of the river or of the precipitation gradient as a driver of population differentiation. Gene flow across the river may be facilitated by its narrow headwaters and may occur as a consequence of river dynamics reducing its effectiveness as a barrier. Also, the dispersal abilities of the species may allow them to overcome the potential barrier that the river represents. As for the ecological barrier, we did not find that it caused any effect on the populations that we studied, probably because climatic differences were not strong enough to have led to population isolation along the MRV; alternatively, it is possible that the time elapsed since the origin of the precipititation gradient has been insufficient to lead to detectable effects on population structure. Our results contrast with work in other regions, which has shown marked genetic structure of vertebrate populations across major Neotropical rivers.
Physical and ecological barriers to dispersal are known to restrict gene flow among populations, ultimately leading to evolutionary divergence and speciation (Haffer 1997, Bohonak 1999, Barraclough and Vogler 2000, Aleixo 2004, Smith et al. 2014). Although geographic isolation by physical barriers likely accounts for a large proportion of speciation events in tropical forest organisms (Moritz et al. 2000, Smith et al. 2014), natural selection in contrasting environments in the absence of physical isolation also might drive speciation and contribute to the buildup of tropical biodiversity (Moritz et al. 2000). However, studies examining the relative roles of physical and geographical barriers as drivers of population differentiation and speciation are scarce (Smith et al. 1997, 2014, Milá et al. 2009, Oliveira et al. 2015, Weir et al. 2015).
Several geographic features of the South American physical landscape contribute to the astounding avian diversity of the Neotropics. Inter-Andean valleys, rivers of the Amazon Basin, and the Andean mountain range are all important barriers to gene flow responsible for isolation and population differentiation in allopatry in Neotropical birds (e.g., Aleixo 2004, Cheviron et al. 2005, Ribas et al. 2012, Gutiérrez-Pinto et al. 2012, Maldonado-Coelho et al. 2013, Smith et al. 2014, Fernandes et al. 2014). A less-studied mechanism of population differentiation in Neotropical birds is environmental variation, which might play a role in restricting gene flow among populations (Rundle and Nosil 2005, Peterson and Nyári 2008, Nosil and Harmon 2009, Rodriguez et al. 2015). For example, spatial variation in food resources (Ryan et al. 2007), vegetation structure (Smith et al. 1997, Nicholls et al. 2006, Kirschel et al. 2011), or biotic and abiotic factors that vary with elevation (Freedman et al. 2010) may promote divergence in continuously distributed populations. The hypothesis of isolation by environment predicts that genetic distances between populations should increase with environmental differences, independent of geographic distance or of the presence of geographic barriers (Wang and Bradburd 2014).
An ideal setting in which to test the relative roles of physical isolation and isolation by environment as drivers of population differentiation would be a landscape in which species occurring on both sides of a geographical barrier are also distributed along an environmental gradient running parallel to the barrier. The Magdalena River Valley (hereafter, MRV) is the largest inter-Andean valley in northern South America, running south–north between the Eastern and Central Cordilleras of the Colombian Andes. The Magdalena River is the largest South American river west of the Andes and may reach >3 km in width near its mouth, thus potentially representing a major barrier for organisms with limited dispersal abilities (Moore et al. 2008). Additionally, the MRV exhibits a steep gradient in annual precipitation, ranging from ∼700 mm in the south to ∼4,000 mm in the north (Figure 1). This gradient results in a marked shift in vegetation, from tropical dry forest in the south to tropical wet forest in the north of the MRV; the transition between habitat types is rather sharp, occurring over ∼60 km (Olson et al. 2001; Figure 1). Thus, the Magdalena River may constitute a physical barrier restricting east–west gene flow between populations located on opposite banks of the river, while varying climatic conditions may promote north–south differentiation of populations of species continuously distributed along its valley. Although the MRV is among the most important geographic and ecological barriers west of the Andes, its role in promoting divergence through vicariant and ecological mechanisms has seldom been proposed or tested (Haffer 1967, Link et al. 2015, Muñoz-Ortiz et al. 2015). This contrasts with ample research on the topic in lowland rivers east of the Andes, several of which are of comparable size to the Magdalena River (e.g., Patton et al. 1994, Bates et al. 2004, Maldonado-Coelho et al. 2013).
In this study, we examined the influence of the MRV on the diversification of lowland organisms by studying geographic variation in 4 species of widely distributed forest birds with presumably varied dispersal abilities. Specifically, we first evaluated whether the Magdalena River acts as a physical barrier for avian populations; this hypothesis predicts restricted gene flow across the river resulting in (1) populations from one bank of the river being more closely related to each other than to any population from the opposite bank (i.e. reciprocal monophyly of populations from separate banks), or (2) greater genetic distances between populations across the river from each other than between populations separated by similar distances on the same river bank. Additionally, we evaluated the extent to which differences in precipitation along the MRV might act as an ecological barrier to gene flow among populations; this hypothesis predicts (1) that populations from one section of the valley (either dry or wet) will be more closely related to each other than to populations from the other section of the valley, or (2) greater genetic distances between populations from different sections of the valley relative to populations separated by similar distances within a section. Because the above hypotheses are not mutually exclusive, we also examined the extent to which physical isolation and ecology may have jointly structured populations of bird species in the region.
Taxon and Geographic Sampling
We selected 15 localities (Figure 1) on both sides of the Magdalena River (8 on the western bank, 7 on the eastern bank) and distributed along the precipitation gradient of the valley (6 in dry forest, 9 in wet forest), in which we collected specimens of 4 locally abundant avian species (Appendix Table 3): Cocoa Woodcreeper (Xiphorhynchus susurrans, Furnariidae [n = 33]), Ochre-bellied Flycatcher (Mionectes oleagineus, Tyrannidae [n = 19]), Sepia-capped Flycatcher (Leptopogon amaurocephalus, Tyrannidae [n = 18]), and Gray-headed Tanager (Eucometis penicillata, Thraupidae [n = 37]). We selected these species because of their different habits, which likely influence their dispersal ability and hence the genetic structure of populations (Burney and Brumfield 2009, Smith et al. 2014, Paz et al. 2015). X. susurrans forages on tree trunks from the understory to the forest canopy for midsized insects and occasionally small vertebrates, and often follows army ant swarms (del Hoyo et al. 2003). M. oleagineus is largely frugivorous but also eats insects and other arthropods, and usually forages in the understory but may forage up to 10 m above the ground (del Hoyo et al. 2004). L. amaurocephalus forages on small arthropods in shady leafy settings, usually within 8 m of the ground, and occasionally eats small fruits (del Hoyo et al. 2004). Finally, E. penicillata feeds mainly on fruits and insects and forages close to the ground (Isler and Isler 1999). Birds were captured using mist nets, prepared as study skins, and deposited in the Museo de Historia Natural ANDES at Universidad de Los Andes in Bogotá, Colombia (Appendix Table 3).
Extraction, Amplification, and Sequencing of DNA
We extracted DNA from pectoral muscle tissue using a phenol chloroform protocol (see Gutiérrez-Pinto et al. 2012 for details). We then amplified the mitochondrial cytochrome b gene (cyt b; 1,056 bp [base pairs] for Cocoa Woodcreeper, 1,027 bp for Ochre-Bellied Flycatcher, 999 bp for Sepia-Capped Flycatcher, and 1,046 bp for Gray-Headed Tanager) using the primer pair L14996/H16064 (Sorenson et al. 1999, Sambrook and Russell 2001). The amplification reactions consisted of 2 μL of template extract (∼50 ng of DNA), 1 μL of 10 mM dNTPs (deoxynucleotide triphosphates), 1.2 μLof each primer (10 mM), 2.5 μL of 10× buffer with 1.5 μL of MgCl2, 0.125 μL Taq DNA polymerase, and 16.5 μL of sterile ddH2O (double-distilled water). We ran PCRs in a PTC-200 Thermal Cycler (MJ Research, Waltham, Massachusetts, USA), beginning with an initial denaturation at 94°C for 2 min, followed by 34 cycles of denaturation at 94°C for 30 s, annealing at 52°C for 30 s, and extension at 72°C for 1 min, with a final extension phase at 72°C for 7 min. PCR products were purified with Exosap-IT (USB Corporation, Cleveland, Ohio, USA), and sequenced in both directions by Macrogen (Seoul, Korea). Sequences were deposited in GenBank (Appendix Table 3).
Phylogenetic and Population Genetic Analyses
For phylogenetic analyses, we enriched our dataset with cyt b sequences available in GenBank (Appendix Table 3) corresponding to individuals from outside the MRV. We determined the models of evolution that best fitted the data using jModelTest 2.1.7 (Posada 2008). We then employed the selected models in Bayesian phylogenetic analyses in the program MrBayes (Ronquist and Huelsenbeck 2003); 4 Monte Carlo Markov Chains were allowed to run for 6 million generations for each species. Chains converged in all cases, as indicated by effective parameter sample sizes >200, mean standard deviation of split frequencies <0.01, and potential scale reduction factors of ∼1. Based on the trees retained, we constructed a 50% majority-rule consensus tree for each species. Outgroups were chosen based on existing phylogenetic information (Jetz et al. 2012): Buff-throated Woodcreeper (Xiphorhynchus guttatus) for Cocoa Woodcreeper, McConnell's Flycatcher (Mionectes macconnelli) for Ochre-bellied Flycatcher and for Sepia-capped Flycatcher, and Black-goggled Tanager (Trichothraupis melanops) for Gray-headed Tanager (Appendix Table 3). In addition, using only individuals captured in the MRV, we constructed median-joining haplotype networks separately for each species using the pegas package (Paradis 2010) for R 3.0.2 (R Core Team 2013).
We used analyses of molecular variance (AMOVA) to determine the extent to which populations were structured by the river barrier or the precipitation gradient. We assigned populations to a side of the river (east or west) and to the type of forest (wet or dry) using the boundaries of the dry and wet forest ecoregions of the MRV (Olson et al. 2001). Based on these divisions, we evaluated the structure among populations from different river banks and/or type of forest, among populations from the same bank and/or type of forest, and within populations (Maldonado-Coelho et al. 2013, Link et al. 2015).
We also tested for isolation by environment by examining the correlation between climatic distance and genetic distance among populations (Wang and Bradburd 2014). For this analysis, we correlated Euclidean distances in annual precipitation among localities based on WorldClim data (Hijmans et al. 2005) with mean Nei's (1972) genetic distance calculated using the R package adegenet (Jombart 2008). Additionally, we examined whether the geographic distance between populations affected the genetic structure of populations within the MRV (i.e. isolation by distance) using a Mantel test that related geographic and genetic distance matrices for each species. Geographic distances were estimated using ArcGIS 10.1 (ESRI, Redlands, California, USA). To test for an interaction between climatic distance and the effect of the river as a barrier, we performed partial Mantel tests using a third matrix in which the distance between populations on different sides of the river was scored as 1 and that between populations on the same bank was scored as 0 (Maldonado-Coelho et al. 2013). Mantel and partial Mantel tests were performed using Pearson's product moment correlation in the Vegan package for R (Oksanen et al. 2012), and significance was determined based on 1,000 permutations of the precipitation distance matrix.
Across our study sites, we observed 6 cyt b haplotypes in X. susurrans (n = 31) and in L. amaurocephalus (n = 18), 3 haplotypes in M. oleagineus (n = 17), and 7 haplotypes in E. penicillata (n = 37). Phylogenetic analyses that included a few samples from outside the MRV revealed somewhat different phylogeographic patterns among our study species. In X. susurrans and L. amaurocephalus we found little geographic structure and shallow divergence, whereas populations of M. oleagineus and E. penicillata showed greater structure and deeper divergence (Figure 2). In the latter 2 species, most samples from the MRV formed a clade separate from other populations, but in no case did we observe the existence of subclades separating populations from the MRV based on river bank or forest type.
Haplotype networks constructed for all species showed no structure among populations, a pattern inconsistent with the hypotheses that the river or the precipitation gradient cause population isolation. In all cases, the most frequently observed haplotypes occurred on both sides of the river and in both types of forest (Figure 3). However, a few rare haplotypes were present exclusively on one side of the river or in one type of forest (Figure 3). Despite this, AMOVAs showed that most of the variation for all species lay within populations (Table 1), and FST values were close to zero, indicating no genetic structure among the populations sampled. Geographic distance, the river barrier, and precipitation distance alone had no effect on the structure of populations within the MRV; only for the Gray-headed Tanager did we find a slight, but significant, positive relationship between precipitation and genetic distances (r = 0.36, P = 0.007), and geographic and genetic distances (r = 0.36, P = 0.006; Table 2, Figure 4). None of the partial tests including river bank as an explanatory variable for genetic distance were significant (Table 2).
Results of analyses of molecular variance (AMOVA) showing that forest type (wet vs. dry) and the Magdalena River, Colombia, did not influence population structure in any of the 4 locally abundant species studied.
P < 0.01; ** P < 0.001.
Results of Mantel tests examining the relationship between genetic distance and geographic and precipitation distances of 4 locally abundant species in the Magdalena River Valley, Colombia. An additional categorical matrix (river) was used in the Mantel tests to evaluate the effect of the Magdalena River on the genetic distances among individuals. If 2 individuals belonged to populations from different banks of the river, the matrix value was entered as 1, and if they were collected from populations on the same side, the matrix value was 0. Relationships were not significant except for Eucometis penicillata, for which genetic distance was weakly correlated with both geographic and environmental distances (significant results are highlighted in bold font). Upper 97.5% Quantile = the 97.5th percentile of the distribution of the correlation coeficient with randomized data.
We found no evidence to support the hypotheses that the Magdalena River or the precipitation gradient along its valley act as barriers to dispersal of individuals among populations of 4 lowland bird species. Our mitochondrial DNA sequence data revealed low overall genetic diversity and lack of population structure, even though some populations were separated by an ∼1-km-wide river and by nearly 700 km and occurred in environments that differed by up to ∼3,000 mm in annual rainfall (Hijmans et al. 2005).
Considering that several rivers in the Neotropical region—some of which are not particularly large—are known to act as geographic barriers for birds (Ribas et al. 2012, Naka et al. 2012, Maldonado-Coelho et al. 2013, Fernandes et al. 2014, Smith et al. 2014) and other vertebrates (Gascon et al. 1996, Fouquet et al. 2012), why is it that it that the Magdalena River does not act as a barrier to gene flow among populations, despite it being the largest river west of the Andes in South America? Our results are not unique to birds, because similar findings (i.e. lack of genetic differentiation across the river and little to no genetic structure along the valley) were also obtained in a recent study on brown spider monkeys (Ateles hybridus; Link et al. 2015). Gene flow across large rivers that may represent insurmountable barriers in their lower reaches may occur readily near their headwaters (Capparella 1988, 1991, Ayres and Clutton-Brock 1992, Peres et al. 1996, Gascon et al. 1998, Gehring et al. 2012, Naka et al. 2012); indeed, a recent study showed that distinct populations of Amazonian birds meet and hybridize in the headwater regions of the Tapajós, a major river known to promote population divergence (Weir et al. 2015). Thus, the lack of population differentiation across the Magdalena River may be explained by dispersal across its narrow headwaters. In addition, the effect of rivers such as the Magdalena as barriers may be transient because they form meanders and change course through time, allowing populations that were isolated in the past to reconnect after reconfigurations of basins. In addition, rivers such as the Magdalena form islands that may serve as stepping-stones, allowing for dispersal (Aleixo 2004, Jackson and Austin 2013, Link et al. 2015). Finally, during dry periods, rivers such as the Magdalena may become greatly reduced in size, allowing for greater connectivity of populations across the river than during wet periods. Nevertheless, some of the Amazonian rivers known to act as barriers to dispersal by birds also form islands or meanders and may fluctuate considerably in water discharge and width (Nanson and Knighton 1996, Mendes et al. 2010, Latrubesse et al. 2013), which suggests that properties of the Magdalena River may not fully account for our results.
Another potential explanation for the lack of genetic structure of populations across the Magdalena River is that our study species may have good dispersal abilities relative to the size of the barrier. A recent study has suggested that the dispersal ability of bird species is one of the most important factors accounting for speciation across geographical barriers, including rivers (Smith et al. 2014; see also Burney and Brumfield 2009, Claramunt et al. 2012). In fact, 2 of the 4 species evaluated in our study (X. susurrans and M. oleagineus) have been shown to be able to potentially overcome water barriers >300 m wide (Moore et al. 2008), and previous work has shown that Amazonian rivers with similar widths to the Magdalena do not represent barriers for other species in the genus Xiphorhynchus (Aleixo 2004). In contrast, examples of differentiation across other Neotropical rivers often involve species with limited dispersal abilities (e.g., trumpeters: Ribas et al. 2012; antbirds: Maldonado-Coelho et al. 2013). Work on species that occur in the MRV and that have lower dispersal abilities (e.g., some members of the Thamnophilidae or Troglodytidae) is necessary to determine whether the Magdalena River may indeed act as a geographic barrier for some birds (Hayes and Sewlal 2004, Moore et al. 2008).
We also failed to find evidence for the precipitation gradient as a factor causing genetic structure of populations along the MRV. Although this may suggest that habitat variation driven by precipitation has no effect on population structure in the region, our results may simply reflect the region's geological and climatic history. Until the Last Glacial Maximum, the MRV is thought to have been covered mostly by dry forest (Haffer 1967), which formed a continuous habitat north to the Caribbean lowlands in northern Colombia and Venezuela. Thus, the precipitation gradient is likely to have a relatively recent origin, with rainforests of the mid-MRV (and their avifaunas) dating to ∼12,000 yr before present (Haffer 1967). Hence, if spatial changes in precipitation indeed represent an ecological barrier to dispersal by our study species, then it is possible that we failed to detect divergence in the genes that we studied because there has not been enough time for changes to accumulate and for populations to achieve significant structure, let alone reciprocal monophyly (Edwards and Beerli 2000).
Alternatively, the precipitation gradient may not represent a barrier to gene flow in neutral mtDNA genes, but may lead to divergence in functionally important traits owing to differential selection in contrasting environments (Smith et al. 1997, Doebeli and Dieckmann 2003, Milá et al. 2007, 2009, McCormack et al. 2008, Oliveira et al. 2015). Studies examining variation in morphological traits and in multiple loci across the genome would shed further light on the role that environmental variation along the MRV may have in driving phenotypic and genetic divergence among populations. In fact, as a follow-up to the present study, we are currently using RADseq (restriction site-associated DNA sequencing) data to explore genome-wide genetic structure in our focal species. Our goals are to evaluate the consistency of the patterns of low genetic structure among populations along the MRV found in this study and to explore the possibility that some functionally important loci might be under divergent selection by extreme differences in the environment despite gene flow in neutral markers.
We thank G. Campuzano, C. García, H. Yara, C. Mendoza, the Pizano family, A. M. Jaramillo, R. N. Rio Claro, A. Link, Biodiversa Colombia, La Mejía, and Fundación Vapores de Magdalena for allowing us to work on their properties. We are grateful to J. L. Parra, J. Llano, E. Yepes, E. A. Hurtado, A. Morales, O. Laverde, S. Chaparro, and S. Arango for invaluable help in the field. Members of the Laboratorio de Biología Evolutiva de Vertebrados assisted with laboratory procedures. We extend our gratitude to J. L. Parra, R. Castañeda, P. Pulgarín, C. Palacios, N. Gutiérrez, G. A. Londoño, and C. Bota for their support and intellectual contribution to our work.
Funding statement: This work was supported by the National Science Foundation (award no. DEB-0841729 to Robb T. Brumfield at Louisiana State University), Explorers Club (Youth Activity Fund grant), National Geographic (Waitts Grant #270-13), Wilson Ornithological Society (Louis Agassiz Fuertes Grant), and the American Museum of Natural History (Frank M. Chapman Grant). None of the funders had any input into the content of the manuscript, and none of the funders required their approval of the manuscript before submission or publication.
Ethics statement: All specimens were collected under permit no. 496 issued on February 19, 2013, by the Colombian Ministerio del Medio Ambiente and the Agencia Nacional de Licencias Ambientales (ANLA).
Author contributions: C.D.C. and J.P.G. conceived the idea and designed the study. J.P.G. and J.S.-H. performed the fieldwork. J.P.G. and J.S.-H. analyzed the data. C.D.C. and J.P.G. contributed substantial materials, resources, or funding. C.D.C., J.P.G., and J.S.-H. wrote the paper.
- Aleixo, A. (2004). Historical diversification of a terra-firme forest bird superspecies: A phylogeographic perspective on the role of different hypotheses of Amazonian diversification. Evolution 58:1303–1317. Google Scholar
- Ayres, J. M., and T. H. Clutton-Brock (1992). River boundaries and species range size in Amazonian primates. The American Naturalist 140:531–537. Google Scholar
- Barraclough, T. G., and A. P. Vogler (2000). Detecting the geographical pattern of speciation from species-level phylogenies. The American Naturalist 155:419–434. Google Scholar
- Bates, J. M., J. Haffer, and E. Grismer (2004). Avian mitochondrial DNA sequence divergence across a headwater stream of the Rio Tapajós, a major Amazonian river. Journal of Ornithology 145:199–205. Google Scholar
- Bohonak, A. J. (1999). Dispersal, gene flow, and population structure. Quarterly Review of Biology 74:21–45. Google Scholar
- Burney, C. W., and R. T. Brumfield (2009). Ecology predicts levels of genetic differentiation in Neotropical birds. The American Naturalist 174: 358–368. Google Scholar
- Capparella, A. P. (1988). Genetic variation in Neotropical birds: Implications for the speciation process. Acta XIX Congressus Internationalis Ornithologici 2:1658–1664. Google Scholar
- Capparella, A. P. (1991). Neotropical avian diversity and riverine barriers. Acta XX Congressus Internationalis Ornithologici 1:307–316. Google Scholar
- Cheviron, Z. A., S. J. Hackett, and A. P. Capparella (2005). Complex evolutionary history of a Neotropical lowland forest bird (Lepidothrix coronata) and its implications for historical hypotheses of the origin of Neotropical avian diversity. Molecular Phylogenetics and Evolution 36:338–357. Google Scholar
- Claramunt, S., E. P. Derryberry, J. V. Remsen, and R. T. Brumfield (2012). High dispersal ability inhibits speciation in a continental radiation of passerine birds. Proceedings of the Royal Society of London, Series B 279:1567–1574. Google Scholar
- del Hoyo, J., A. Elliott, and D. A. Christie (Editors)(2003). Handbook of the Birds of the World, Volume 8: Broadbills to Tapaculos. Lynx Edicions, Barcelona, Spain. Google Scholar
- del Hoyo, J., A. Elliott, and D. A. Christie (Editors)(2004). Handbook of the Birds of the World, Volume 9: Cotingas to Pipits and Wagtails. Lynx Edicions, Barcelona, Spain. Google Scholar
- Doebeli, M., and U. Dieckmann (2003). Speciation along environmental gradients. Nature 421:259–264. Google Scholar
- Edwards, S. V., and P. Beerli (2000). Perspective: Gene divergence, population divergence, and the variance in coalescence time in phylogeographic studies. Evolution 54:1839–1854. Google Scholar
- Fernandes, A. M., M. Cohn-Haft, T. Hrbek, and I. P. Farias (2014). Rivers acting as barriers for bird dispersal in the Amazon. Revista Brasileira de Ornitologia 22:363–373. Google Scholar
- Fouquet, A., J.-B. Ledoux, V. Dubut, B. P. Noonan, and I. Scotti (2012). The interplay of dispersal limitation, rivers, and historical events shapes the genetic structure of an Amazonian frog. Biological Journal of the Linnean Society 106:356–373. Google Scholar
- Freedman, A. H., H. A. Thomassen, W. Buermann, and T. B. Smith (2010). Genomic signals of diversification along ecological gradients in a tropical lizard. Molecular Ecology 19:3773–3788. Google Scholar
- Gascon, C., S. C. Lougheed, and J. P. Bogart (1996). Genetic and morphological variation in Vanzolinius discodactylus: A test for the river hypothesis of speciation. Biotropica 28:376–387. Google Scholar
- Gascon, C., S. C. Lougheed, and J. P. Bogart (1998). Patterns of genetic population differentiation in four species of Amazonian frogs: A test of the Riverine Barrier Hypothesis. Biotropica 30:104–119. Google Scholar
- Gehring, P. S., M. Pabijan, J. E. Randrianirina, F. Glaw, and M. Vences (2012). The influence of riverine barriers on phylogeogrpahic patterns of Malagasy reed frogs (Heterixalus). Molecular Phylogenetics and Evolution 64:618–632. Google Scholar
- Gutiérrez-Pinto, N., A. M. Cuervo, J. Miranda, J. L. Pérez-Emán, R. T. Brumfield, and C. D. Cadena (2012). Non-monophyly and deep genetic differentiation across low-elevation barriers in a Neotropical montane bird (Basileuterus tristriatus; Aves: Parulidae). Molecular Phylogenetics and Evolution 64:156–165. Google Scholar
- Haffer, J. (1967). Speciation in Colombian Forest Birds West of the Andes. American Museum Novitates 2294. Google Scholar
- Haffer, J. (1997). Alternative models of vertebrate speciation in Amazonia: An overview. Biodiversity and Conservation 476:451–476. Google Scholar
- Hayes, F. E., and J.-A. N. Sewlal (2004).The Amazon River as a dispersal barrier to passerine birds: Effects of river width, habitat and taxonomy. Journal of Biogeography 31:1809–1818. Google Scholar
- Hijmans, R. J., S. E. Cameron, J. L. Parra, P. G. Jones, and A. Jarvis (2005). Very high resolution interpolated climate surfaces for global land areas. International Journal of Climatology 25:1965–1978. Google Scholar
- Isler, M. L., and P. R. Isler (1999). The Tanagers: Natural History, Distribution, and Identification. Smithsonian Institution Press, Washington, DC, USA. Google Scholar
- Jackson, N. D., and C. C. Austin (2013). Testing the role of meander cutoff in promoting gene flow across a riverine barrier in ground skinks (Scincella lateralis). PLOS One 8:e62812. 10.1371/journal.pone.0062812 Google Scholar
- Jetz, W., G. H. Thomas, J. B. Joy, K. Hartmann, and A. O. Mooers (2012). The global diversity of birds in space and time. Nature 491:444–448. Google Scholar
- Jombart, T. (2008). adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24:1403–1405. Google Scholar
- Kirschel, A. N. G., H. Slabbekoorn, D. T. Blumstein, R. E. Cohen, S. R. de Kort, W. Buermann, and T. B. Smith (2011). Testing alternative hypotheses for evolutionary diversification in an African songbird: Rainforest refugia versus ecological gradients. Evolution 65:3162–3174. Google Scholar
- Latrubesse, E. M., C. Bonthius, J. D. Abad, J. Stevaux, N. Filizola, and C. E. Frias (2013). Morphodynamics and anabranching patterns generated in the Madeira River, Brazil. In AGU Fall Meeting Abstracts, abstract #EP33C-0926. http://adsabs.harvard.edu/abs/2013AGUFMEP33C0926L Google Scholar
- Link, A., L. M. Valencia, L. N. Céspedes, L. D. Duque, C. D. Cadena, and A. Di Fiore (2015). Phylogeography of the critically endangered brown spider monkey (Ateles hybridus): Testing the riverine barrier hypothesis. International Journal of Primatology 36:530–547. Google Scholar
- Maldonado-Coelho, M., J. G. Blake, L. F. Silveira, H. Batalha-Filho, and R. E. Ricklefs (2013). Rivers, refuges and population divergence of fire-eye antbirds (Pyriglena) in the Amazon Basin. Journal of Evolutionary Biology 26:1090–1107. Google Scholar
- McCormack, J. E., A. T. Peterson, E. Bonaccorso, and T. B. Smith (2008). Speciation in the highlands of Mexico: Genetic and phenotypic divergence in the Mexican Jay (Aphelocoma ultramarina). Molecular Ecology 17:2505–2521. Google Scholar
- Mendes, D., M. L. Assine, and F. A. Corradini (2010). Fluvial styles of the Negro River on the Pantanal wetland, Brazil [Abstract]. InIGCP 582—Tropical Rivers: Hydro-Physical Processes, Impacts, Hazards and Management ( E. Latrubesse, J. C. Stevaux, and R. Sinha, Convenors).UNESCO International Geoscience Programme, Paris, France. http://www.igcp582.uem.br/ Google Scholar
- Milá, B., J. E. McCormack, G. Castañeda, R. K. Wayne, and T. B. Smith (2007). Recent postglacial range expansion drives the rapid diversification of a songbird lineage in the genus Junco. Proceedings of the Royal Society of London, Series B 274:2653–2660. Google Scholar
- Milá, B., R. K. Wayne, P. Fitze, and T. B. Smith (2009). Divergence with gene flow and fine-scale phylogeographical structure in the Wedge-billed Woodcreeper, Glyphorynchus spirurus, a Neotropical rainforest bird. Molecular Ecology 18:2979–2995. Google Scholar
- Moore, R. P., W. D. Robinson, I. J. Lovette, and T. R. Robinson (2008). Experimental evidence for extreme dispersal limitation in tropical forest birds. Ecology Letters 11:960–968. Google Scholar
- Moritz, C., J. L. Patton, C. J. Schneider, and T. B. Smith (2000). Diversification of rainforest faunas: An integrated molecular approach. Annual Review of Ecology and Systematics 31:533–563. Google Scholar
- Muñoz-Ortiz, A., A. A. Velásquez-Álvarez , C. E. Guarnizo, and A. J. Crawford (2015). Of peaks and valleys: Testing the roles of orogeny and habitat heterogeneity in driving allopatry in mid-elevation frogs (Aromobatidae: Rheobates) of the northern Andes. Journal of Biogeography 42:193–205. Google Scholar
- Naka, L. N., C. L. Bechtoldt, L. M. P. Henriques, and R. T. Brumfield (2012). The role of physical barriers in the location of avian suture zones in the Guiana Shield, northern Amazonia. The American Naturalist 179:E115–E132. Google Scholar
- Nanson, G. C., and A. D. Knighton (1996). Anabranching rivers: Their cause, character and classification. Earth Surface Processes and Landforms 21:217–239. Google Scholar
- Nei, M. (1972). Genetic distance between populations. The American Naturalist 106:283–292. Google Scholar
- Nicholls, J. A., J. J. Austin ., C. Moritz, and A. W. Goldizen (2006). Genetic population structure and call variation in a passerine bird, the Satin Bowerbird, Ptilonorhynchus violaceus. Evolution 60:1279–1290. Google Scholar
- Nosil, P., and L. J. Harmon (2009). Niche dimensionality and ecological speciation. InSpeciation and Patterns of Diversity ( R. Butlin, Editor). Cambridge University Press, Cambridge, UK. pp. 127–154. Google Scholar
- Oksanen, J., F. G. Blanchet, R. Kindt, P. Legendre, P. R. Minchin, R. B. O'Hara, G. L. Simpson, P. Solymos, M. H. H. Stevens, and H. Wagner (2012). vegan: Community Ecology Package. R package version 2.0-5. http://CRAN.R-project.org/package=vegan Google Scholar
- Oliveira, E. F., M. Gehara, V. A. São-Pedro, X. Chen, E. A. Myers, F. T. Burbrink, D. O. Mesquita, A. A. Garda, G. R. Colli, M. T. Rodrigues, F. J. Arias, et al. (2015). Speciation with gene flow in whiptail lizards from a Neotropical xeric biome. Molecular Ecology 24:5957–5975. Google Scholar
- Olson, D. M., E. Dinerstein, E. D. Wikramanayake, N. D. Burgess, G. V. N. Powell, E. C. Underwood, J. A. D'Amico, I. Itoua, H. E. Strand, J. C. Morrison, C. J. Loucks, et al. (2001). Terrestrial ecoregions of the world: A new map of life on earth. BioScience 51:933–938. Google Scholar
- Paradis, E. (2010). pegas: an R package for population genetics with an integrated–modular approach. Bioinformatics 26:419–420. Google Scholar
- Patton, J. L., M. N. F. da Silva, and J. R. Malcolm (1994). Gene genealogy and differentiation among arboreal spiny rats (Rodentia: Echimyidae) of the Amazon Basin: A test of the riverine barrier hypothesis. Evolution 48:1314–1323. Google Scholar
- Paz, A., R. Ibáñez, K. R. Lips, and A. J. Crawford (2015). Testing the role of ecology and life history in structuring genetic variation across a landscape: A trait-based phylogeographic approach. Molecular Ecology 24:3723–3737. Google Scholar
- Peres, C. A., J. L. Patton, and M. N. F. da Silva (1996). Riverine barriers and gene flow in Amazonian saddle-back tamarins. Folia Primatologica 67:113–124. Google Scholar
- Peterson, A. T., and Á. S. Nyári (2008). Ecological niche conservatism and Pleistocene refugia in the Thrush-like Mourner, Schiffornis sp., in the Neotropics. Evolution 62:173–183. Google Scholar
- Posada, D. (2008). jModelTest: Phylogenetic model averaging. Molecular Biology and Evolution 25:1253–1256. Google Scholar
- R Core Team (2013). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/ Google Scholar
- Ribas, C. C., A. Aleixo, A. C. R. Nogueira, C. Y. Miyaki, and C. Cracraft (2012). A palaeobiogeographic model for biotic diversification within Amazonia over the past three million years. Proceedings of the Royal Society of London, Series B 279:681–689. Google Scholar
- Rodriguez, A., M. Börner, M. Pabijan, M. Gehara, C. F. B. Haddad, and M. Vences (2015). Genetic divergence in tropical anurans: Deeper phylogeographic structure in forest specialists and in topographically complex regions. Evolutionary Ecology 29:765–785. Google Scholar
- Ronquist, F., and J. P Huelsenbeck (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. Google Scholar
- Rundle, H. D., and P. Nosil (2005). Ecological speciation. Ecology Letters 8:336–352. Google Scholar
- Ryan, P. G., P. Bloomer, C. L Moloney, T. J. Grant, and W. Delport (2007). Ecological speciation in South Atlantic island finches. Science 315:1420–1423. Google Scholar
- Sambrook, J. J., and D. W. Russell (2001). Molecular Cloning: A Laboratory Manual, third edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA. Google Scholar
- Smith, B. T., J. E. McCormack, A. M. Cuervo, M. J. Hickerson, A. Aleixo, C. D. Cadena, J. Pérez-Emán, C. W. Burney, X. Xie, M. G. Harvey, B. C. Faircloth, et al. (2014). The drivers of tropical speciation. Nature 515:406–409. Google Scholar
- Smith, T. B., R. K. Wayne, D. J. Girman, and M. W. Bruford (1997). A role for ecotones in generating rainforest biodiversity. Science 276:1855–1857. Google Scholar
- Sorenson, M. D., J. C. Ast, D. E. Dimcheff, T. Yuri, and D. P. Mindell (1999). Primers for a PCR-based approach to mitochondrial genome sequencing in birds and other vertebrates. Molecular Phylogenetics and Evolution 12:105–114. Google Scholar
- Wang, I. J., and G. S. Bradburd (2014). Isolation by environment. Molecular Ecology 23:5649–5662. Google Scholar
- Weir, J. T., M. S. Faccio, P. Pulido-Santacruz, A. O. Barrera-Guzmán, and A. Aleixo (2015). Hybridization in headwater regions, and the role of rivers as drivers of speciation in Amazonian birds. Evolution 69:1823–1834. Google Scholar
APPENDIX TABLE 3.
Detailed information on sampling localities, species sampled, UNIANDES-O catalog numbers (Catalog), geographic coordinates (latitude and longitude), side of the Magdalena River (Side; E = east bank, W = west bank), mean annual precipitation in millimeters at each sampling locality (Prec), and GenBank accession numbers (GenBank) for each specimen used in our analyses of diversification of lowland birds in the Magdalena River Valley, Colombia. GenBank accession numbers of sequences generated during this study are highlighted in bold font. NA denotes individuals collected outside the Magdalena River Valley. These samples were used for phylogenetic analyses but were excluded from the construction of haplotype networks and from population genetics analyses. Species are listed in taxonomic order and then by GenBank accession number. Species abbreviations: XISU = Xiphorhynchus susurrans, XIGU = Xiphorhynchus guttatus, PSSI = Pseudotriccus simplex, MIOL = Mionectes oleagineus, MIMA = Mionectes macconnelli, LEAM = Leptopogon amaurocephalus, TRME = Trichothraupis melanops, and EUPE = Eucometis penicillata.