Beetles in the genus Acmaeodera were collected in southeastern Alberta, Canada. These did not fit the descriptions of the species reported to occur. Comparisons of structural characters and geographic ranges of species of several Acmaeodera species, A. diffusa Barr, A. idahoensis Barr, A. pulchella (Herbst), A. mixta LeConte, and A. immaculata Horn revealed that only A. immaculata is represented in Alberta, which represents a new record for Canada.