We present an updated checklist of the aquatic adephagan Coleoptera collected in northern Tunisia over the course of a year (from May 2005 to April 2006). It includes a total of 57 species (three Gyrinidae, six Haliplidae, one Paelobiidae, one Noteridae, and 46 Dytiscidae). Hydroglyphus major (Sharp, 1882) is recorded for the first time from Tunisia. The distributions of the species in Tunisia and the rest of world is discussed. A list of adephagan species recorded from Tunisia is included.
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