Two new species of Anticyphon Ruta are described from Tucumán in northern Argentina and Azuay Province in southern Ecuador. The Argentine record greatly extends the known geographical range of the genus. The female of Anticyphon was known only for a single species, A. oyonensis Ruta. Anticyphon lescheni Ruta, new species, is the second species of the genus with a known female. In the case of both species, females have five vaginal sclerites and elongated, lanceolate bursal sclerites. It can be expected that more undescribed species of Anticyphon occur in high altitude Andean habitats.