We report the step-by-step process of developing de novo microsatellite (SSR) loci in two Loxosceles spider species. We used reads obtained with next-generation sequencing (Roche 454) to select hundreds of potentially-amplifiable SSRs. After testing amplification and cross-amplification, we characterized 18 SSRs, 11 of which were polymorphic in Loxosceles rufescens (Dufour 1820) and seven of which were polymorphic in L. sp. Fuerteventura - Lanzarote. This method is a relatively fast and economic procedure for the development of fast-evolving nuclear markers in spiders.
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1 November 2014
Development of novel microsatellite markers for the spider genus Loxosceles (Sicariidae) using next-generation sequencing
Enric Planas,
Laia Bernaus,
Carles Ribera
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The Journal of Arachnology
Vol. 42 • No. 3
November 2014
Vol. 42 • No. 3
November 2014
454
Canary Islands
cross-amplification
Mediterranean
nuclear markers