Four new genera of funnel-web spiders of the family Agelenidae C.L. Koch, 1837 from the Baja California Peninsula, Mexico are described and illustrated. Bajacalilena gen. nov. is represented by B. bolzerni sp. nov. and B. clarki sp. nov. Cabolena gen. nov. is represented by C. huiztocatl sp. nov., C. kosatli sp. nov., and C. sotol sp. nov. Callidalena gen. nov. is represented by C. quintin sp. nov. and C. tijuana sp. nov. Lagunella gen. nov. is represented by L. guaycura sp. nov. Additionally, the males of Calilena angelena Chamberlin & Ivie, 1941, Hololena septata Chamberlin & Ivie, 1942, and Rothilena sudcaliforniensis Maya-Morales & Jiménez, 2013 are described for the first time. New records for C. angelena, H. septata, R. cochimi Maya-Morales & Jiménez, 2013, R. pilar Maya-Morales & Jiménez, 2013, and R. sudcaliforniensis are also provided. Molecular analysis of a fragment of cytochrome c oxidase subunit I (COI) provides support for the monophyly of the new genera and facilitated sorting of conspecific males and females. Finally, an identification key to native Nearctic and Neotropical genera of the subfamily Ageleninae is provided.