The spider family Trechaleidae includes 17 genera and 131 species distributed throughout the Neotropical region. Most of them are semiaquatic spiders living adjacent to streams and rivers. Very few species of the family have been studied for their natural history or behavior; however four genera (Paratrechalea Carico, 2005, Trechalea Thorell, 1869, Paradossenus F. O. Pickard-Cambridge, 1903 and Trechaleoides Carico, 2005) are already known to have males that offer wrapped nuptial gifts to females. Here we describe a new species from the genus Enna O. Pickard-Cambridge, 1897, namely E. gloriae sp. nov. from Quindío, Colombia. We additionally report the presence of males of this species offering nuptial gifts to females during courtship, being the first record of this sexual trait for the genus.