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1 January 2000 Identification of Proteins in Encephalitozoon intestinalis, a Microsporidian Pathogen of Immunocompromised Humans: An Immunoblotting and Immunocytochemical Study
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Abstract

Microsporidia are unicellular and obligate intracellular spore-forming parasites. The spore inoculates the host cell with its non-motile infectious content, the sporoplasm, by way of the polar tube–the typical invasive apparatus of the microsporidian spore. Molecules involved in host cell invasion were investigated in Encephalitozoon intestinalis. Mouse polyclonal and monoclonal antibodies were raised against spore proteins and their reactivity was tested by Western-blotting and immunolocalization techniques, including electron and confocal microscopy. The antibodies thus generated could be divided into two major groups. One group reacted to the surface of the parasite at different developmental stages, mostly presporous stages and mature spores, whereas the other group recognized the polar tube. Of the antibodies reacting to the spore wall, one identified an exospore protein at 125 kDa while all others recognized a major doublet at 55–60 kDa, and minor proteins present at the surface of sporogonic stages and in the endospore. All antibodies recognizing spore wall proteins reacted also to the material forming septa in the parasitophorous vacuole. A major polar tube protein at 60 kDa was identified by another group of antibodies.

Odile Prigneau, Abderrahim Achbarou, Nicolas Bouladoux, Dominique Mazier, and I. S. A. B. E. LLE. Desportes-Livage "Identification of Proteins in Encephalitozoon intestinalis, a Microsporidian Pathogen of Immunocompromised Humans: An Immunoblotting and Immunocytochemical Study," The Journal of Eukaryotic Microbiology 47(1), 48-56, (1 January 2000). https://doi.org/10.1111/j.1550-7408.2000.tb00010.x
Received: 28 May 1999; Accepted: 3 August 1999; Published: 1 January 2000
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