1 March 2000 conZA8 Encodes an Abundant Protein Targeted to the Developing Macronucleus in Euplotes crassus
Mary Ellen Jacobs, Zhong Ling, Lawrence A. Klobutcher
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During macronuclear development in the ciliate Euplotes crassus, micronuclear-derived chromosomes undergo a series of rearrangements that include polytenization, DNA splicing, chromosome fragmentation, and telomere addition and processing. Although cis-acting signals that may function in the regulation of these events have been characterized, the proteins that mediate these events have not yet been identified. To identify development-specific factors that may be involved in DNA rearrangement, we previously isolated clones of a number of genes that are expressed only during early macronuclear development. Here, we report the genomic and cDNA sequences of one of these genes, conZA8. The analysis indicates that the conZA8 gene encodes a novel, 468-amino acid, proline-rich protein. Antibodies were raised against both a recombinant form of the conZA8 protein and an internal peptide. Immunoblotting and immunofluorescence analyses indicated that the conZA8 protein is highly abundant, expressed only during the polytene chromosome stage of macronuclear development, and localized to the developing macronucleus. Possible functions of the conZA8 protein are discussed.

Mary Ellen Jacobs, Zhong Ling, and Lawrence A. Klobutcher "conZA8 Encodes an Abundant Protein Targeted to the Developing Macronucleus in Euplotes crassus," The Journal of Eukaryotic Microbiology 47(2), 105-115, (1 March 2000). https://doi.org/10.1111/j.1550-7408.2000.tb00019.x
Received: 6 May 1999; Accepted: 4 October 1999; Published: 1 March 2000

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Anomalous protein migration
DNA rearrangement
hypotrichous ciliate
PEST sequence
polytene chromosomes
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