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1 July 2000 Experimental Amebiasis: Immunohistochemical Study of Immune Cell Populations
Prabir K. Ghosh, Javier Ventura, Sadhna Gupta, Jesus Serrano, Victor Tsutsumi, Librado Ortiz-Ortiz
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Distribution of immune cell populations was studied in a C3H/HeJ mouse model of intestinal amebiasis from 5 to 60 days post-inoculation with Entamoeba histolytica, using immunoperoxidase techniques. At various time intervals, the ceca from mice were fixed in 10% formalin, dehydrated, embedded and sectioned at 5 μm. Sections were incubated with conjugated peroxidase-labelled antibodies to mouse IgA, IgM, and IgG. Color was developed with 3, 3′-diaminobenzidine tetrahydrochloride (DAB)/H2O2 solution. CD3, CD4, and CD8 cells, as well as neutrophils were detected by reacting with biotin-conjugated anti-mouse CD3, CD4, CD8, and CD11 monoclonal antibodies, followed by their incubation with avidin-peroxidase and color development with DAB/H2O2 solution. Erythrocin B and toluidine blue were used to stain eosinophils and Mast cells, respectively. It was observed that the IgA plasma cell was the dominating immune cell present in the mucosa, although eosinophils, neutrophils, CD3 , CD4 , CD8 , IgM , IgG cells and Mast cells were also seen. Results of this study suggest that infiltration of immune cells at the mucosal surface during intestinal amebiasis might be important in the defense against this parasite.

Prabir K. Ghosh, Javier Ventura, Sadhna Gupta, Jesus Serrano, Victor Tsutsumi, and Librado Ortiz-Ortiz "Experimental Amebiasis: Immunohistochemical Study of Immune Cell Populations," The Journal of Eukaryotic Microbiology 47(4), 395-399, (1 July 2000).
Received: 26 January 1999; Accepted: 23 March 2000; Published: 1 July 2000

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Entamoeba histolytica
immuno-histochemical staining
mucosal surface
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