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1 September 2000 Responses of Entamoeba invadens to Heat Shock and Encystation are Related
Jessica Field, Katrina Van Dellen, Sudip K. Ghosh, John Samuelson
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Abstract

An Entamoeba invadens gene encoding a homologue of BiP/GRP78, a 70-kDa heat shock protein or chaperonin was cloned. The predicted E. invadens BiP contained an ATP-binding site, a substrate-recognition domain, and a carboxy-terminal KDEL-peptide. Messenger RNAs of E. invadens for BiP, for a 70-kDa heat shock cognate, for a cyst wall glycoprotein (Jacob), and for chitinase were all induced by heat shock and by encystation medium. The presence of Jacob in heat-shocked amebae was confirmed by confocal microscopy and suggests that heat shock and encystation responses in E. invadens are related.

Jessica Field, Katrina Van Dellen, Sudip K. Ghosh, and John Samuelson "Responses of Entamoeba invadens to Heat Shock and Encystation are Related," The Journal of Eukaryotic Microbiology 47(5), 511-514, (1 September 2000). https://doi.org/10.1111/j.1550-7408.2000.tb00083.x
Received: 12 January 2000; Accepted: 12 June 2000; Published: 1 September 2000
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KEYWORDS
BiP/GRP78
chitinase
cyst wall protein
Hsc70
KDEL-peptide
phylogeny
signal-peptide
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