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1 November 2000 The SSU rDNA Coding Region of a Filose Amoeba Contains a Group I Intron Lacking the Universally Conserved G at the 3′-Terminus
DEBASHISH BHATTACHARYA, MARIANA C. OLIVEIRA
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Abstract

We sequenced small subunit ribosomal DNA (rDNA) PCR-fragments of sizes 2.3 kb and 2.9 kb isolated from a culture of the red alga, Porphyra spiralis var. spiralis. Phylogenetic analysis of the 2.3-kb fragment showed that it encoded the sequence of a contaminant filose amoeba. The Nuclearia-like amoeba (named strain N-Por) was identified with scanning electron microscopy. Its rDNA sequence was positioned with strong bootstrap support within a diverse protist assemblage that includes filose amoebae, chlorarachniophytes, cercomonads, and Plasmodiophora brassicae. The rDNA of N-Por contained a group I intron at the conserved 943 position that remarkably, had a U at the 3′-terminus rather than the universally conserved G.

DEBASHISH BHATTACHARYA and MARIANA C. OLIVEIRA "The SSU rDNA Coding Region of a Filose Amoeba Contains a Group I Intron Lacking the Universally Conserved G at the 3′-Terminus," The Journal of Eukaryotic Microbiology 47(6), 585-589, (1 November 2000). https://doi.org/10.1111/j.1550-7408.2000.tb00094.x
Received: 15 May 2000; Accepted: 3 August 2000; Published: 1 November 2000
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