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1 July 2001 Genetic Diversity in the Microsporidian Encephalitozoon hellem Demonstrated by Pulsed-Field Gel Electrophoresis
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Abstract

Encephalitozoon hellem is a microsporidian species responsible for opportunistic infections in AIDS patients. Use of a novel chitinase-based method allowed unsheared chromosomal DNA to be recovered from eleven E. hellem isolates derived from three geographic regions. All isolates were typed by 18S rDNA sequencing, which showed that they belonged to internal transcribed spacer type 1. After ethidium bromide staining, pulsed-field gel electrophoresis (PFGE) analysis discriminated two new karyotypes comprising 7 and 8 chromosomal bands respectively, ranging in size from 205- to 272-kb pairs. Genomic size was estimated to be 2.39 Mb. Our data indicate PFGE is useful for typing E. hellem and confirms genetic diversity among E. hellem genotypes.

SEBASTIEN DELARBRE, SIMONETTA GATTI, MASSIMO SCAGLIA, and MICHEL DRANCOURT "Genetic Diversity in the Microsporidian Encephalitozoon hellem Demonstrated by Pulsed-Field Gel Electrophoresis," The Journal of Eukaryotic Microbiology 48(4), 471-474, (1 July 2001). https://doi.org/10.1111/j.1550-7408.2001.tb00181.x
Received: 26 September 2000; Accepted: 28 March 2001; Published: 1 July 2001
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