We employed an improved fixation procedure for electron microscopy using ruthenium red, and found a bundle of contractile tubules inside the axopodia of the heliozoon Actinophrys sol. Upon food uptake, the tubules shorten and transform into a mass of small granules when rapid axopodial contraction occurs, suggesting that these structures are involved in the process of axopodial contraction. The relationship between transformation of the contractile tubules and accompanying disassembly of the axonemal microtubules was studied by examining the ultrastructure of the contractile tubules after disassembly of the microtubules was artificially induced by cold or colchicine treatment. Granulation of the contractile tubules was induced by cold but not by colchicine treatment. During recovery from cold treatment, granular forms of the contractile tubules became re-elongated and their initial tubular appearance was restored. These results suggest that the contractile tubules in heliozoon axopodia play a role in repetitive cytoplasmic contraction.
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1 September 2001
The Ultrastructure of Contractile Tubules in the Heliozoon Actinophryssol and Their Possible Involvement in Rapid Axopodial Contraction
EIJI KINOSHITA,
YOSHINOBU SHIGENAKA,
TOSHINOBU SUZAKI
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The Journal of Eukaryotic Microbiology
Vol. 48 • No. 5
September 2001
Vol. 48 • No. 5
September 2001
Axopodium
Ca2 -contraction
colchicine
electron microscopy