Presence of Glyoxylate Cycle Enzymes in the Mitochondria of Euglena gracilis

KOUKI ONO; MAKI KONDO; TETSUAKI OSAFUNE; KAZUTAKA MIYATAKE; HIROSHI INUI; SHOZABURO KITAOKA; MIKIO NISHIMURA; YOSHIHISA NAKANO

doi:10.1111/j.1550-7408.2003.tb00239.x

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1 March 2003 Presence of Glyoxylate Cycle Enzymes in the Mitochondria of Euglena gracilis

KOUKI ONO, MAKI KONDO, TETSUAKI OSAFUNE, KAZUTAKA MIYATAKE, HIROSHI INUI, SHOZABURO KITAOKA, MIKIO NISHIMURA, YOSHIHISA NAKANO

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The J. of Eukaryotic Microbiology, 50(2):92-96 (2003). https://doi.org/10.1111/j.1550-7408.2003.tb00239.x

Abstract

Isocitrate lyase and malate synthase are specific enzymes of the glyoxylate cycle, used here as glyoxysomal markers. Both enzymes were found in the mitochondrial fraction after organelle fractionation by isopycnic centrifugation. Electron microscopy of this fraction indicated that mitochondria were the only recognizable organelles. Using an immunogold labeling method with anti- (malate synthase) antiserum, the only organelles stained in cells were the mitochondria. These results show that the glyoxylate cycle is present in mitochondria in Euglena.

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KOUKI ONO, MAKI KONDO, TETSUAKI OSAFUNE, KAZUTAKA MIYATAKE, HIROSHI INUI, SHOZABURO KITAOKA, MIKIO NISHIMURA, and YOSHIHISA NAKANO "Presence of Glyoxylate Cycle Enzymes in the Mitochondria of Euglena gracilis," The Journal of Eukaryotic Microbiology 50(2), 92-96, (1 March 2003). https://doi.org/10.1111/j.1550-7408.2003.tb00239.x

Received: 18 June 2002; Accepted: 11 December 2002; Published: 1 March 2003

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