Structure of N-glycosidic Carbohydrates of Secretory Proteins of Tetrahymena thermophila

BURKHARD BECKER; MATTHIAS RÜSING

doi:10.1111/j.1550-7408.2003.tb00125.x

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BURKHARD BECKER,¹ MATTHIAS RÜSING²

¹aBotanisches Institut, Universität zu Köln, Gyrhofstr. 15, 50931 Köln, Germany
²bNutrinova Nutrition Specialties & Food Ingredients GmbH, Protos-Biotech, Industriepark Höchst, D-65

Abstract

Glycoproteins secreted by Tetrahymena into the culture medium were isolated and the N-glycosidic oligosaccharides analyzed using lectin blots and fluorophore-assisted carbohydrate gel electrophoresis (FACE). Lectin blots showed that the glycoproteins secreted by Tetrahymena contain only N-glycosidic structures of the high mannose type. Further analysis using the FACE technology revealed the presence of four different N-glycosidic structures differing only in the number of mannose residues attached to the core chitobiose unit.

Citation Download Citation

BURKHARD BECKER and MATTHIAS RÜSING "Structure of N-glycosidic Carbohydrates of Secretory Proteins of Tetrahymena thermophila," The Journal of Eukaryotic Microbiology 50(4), 235-239, (1 July 2003). https://doi.org/10.1111/j.1550-7408.2003.tb00125.x

Received: 27 November 2002; Accepted: 22 March 2003; Published: 1 July 2003