Immunocompetent Infants as a Human Reservoir for Pneumocystis jirovecii: Rapid Screening by Non-Invasive Sampling and Real-Time PCR at the Mitochondrial Large Subunit rRNA Gene

ANNE TOTET; LEILA MELIANI; PHILIPPE LACUBE; JEAN CLAUDE PAUTARD; CHRISTIAN RACCURT; PATRICIA ROUX; GILLES NEVEZ

doi:10.1368/1066-5234(2003)050[0668:IIAAHR]2.0.CO;2

1 November 2003 Immunocompetent Infants as a Human Reservoir for Pneumocystis jirovecii: Rapid Screening by Non-Invasive Sampling and Real-Time PCR at the Mitochondrial Large Subunit rRNA Gene

ANNE TOTET, LEILA MELIANI, PHILIPPE LACUBE, JEAN CLAUDE PAUTARD, CHRISTIAN RACCURT, PATRICIA ROUX, GILLES NEVEZ

Author Affiliations +

The J. of Eukaryotic Microbiology, 50(6):668-669 (2003). https://doi.org/10.1368/1066-5234(2003)050[0668:IIAAHR]2.0.CO;2

Abstract

We tested a real-time PCR assay targeting the Pneumocystis jirovecii mitochondrial large subunit rRNA gene on 240 archival nasopharyngeal aspirates from non-immunosuppressed infants. The sensitivity of this assay appeared close to that of a conventional nested-PCR assay targeting the same locus. Because of its one-step procedure, and its sensitivity and rapidity, the real-time PCR assay is particularly suitable for screening individuals parasitized by P. jirovecii within large populations.

Citation Download Citation

ANNE TOTET, LEILA MELIANI, PHILIPPE LACUBE, JEAN CLAUDE PAUTARD, CHRISTIAN RACCURT, PATRICIA ROUX, and GILLES NEVEZ "Immunocompetent Infants as a Human Reservoir for Pneumocystis jirovecii: Rapid Screening by Non-Invasive Sampling and Real-Time PCR at the Mitochondrial Large Subunit rRNA Gene," The Journal of Eukaryotic Microbiology 50(6), 668-669, (1 November 2003). https://doi.org/10.1368/1066-5234(2003)050[0668:IIAAHR]2.0.CO;2

Published: 1 November 2003

KEYWORDS/PHRASES

PUBLICATION TITLE:

COLLECTION TITLE:

PUBLICATION YEARS