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1 March 2004 The Epiplasm Gene EPC1 Influences Cell Shape and Cortical Pattern in Tetrahymena thermophila
NORMAN E. WILLIAMS
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Abstract

The cortical protein Epc1p is the most abundant protein in the membrane skeleton, or epiplasm, of Tetrahymena thermophila. A partial sequence of the EPC1 gene was obtained and used to obtain a knockout construct that was successful in transforming Tetrahymena thermophila cells. The results support the conclusion that Epc1p influences cell shape and the fidelity of cortical development. It was further observed that this protein is transferred from plus to minus cells during conjugation, and that the imported protein is assembled into the epiplasm of the recipient cell in a discreet series of steps.

NORMAN E. WILLIAMS "The Epiplasm Gene EPC1 Influences Cell Shape and Cortical Pattern in Tetrahymena thermophila," The Journal of Eukaryotic Microbiology 51(2), 201-206, (1 March 2004). https://doi.org/10.1111/j.1550-7408.2004.tb00546.x
Received: 5 September 2003; Accepted: 1 November 2003; Published: 1 March 2004
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KEYWORDS
Conjugation
cortical development
EPC1
epiplasm
gene knockout
membrane skeleton
Tetrahymena
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