Campylocentrum includes over 70 species distributed throughout tropical America. In this paper a synopsis of this genus in Colombia is presented. The occurrence of 16 species of Campylocentrum in this country was confirmed. Morphological characteristics of all the species are presented and illustrated by line drawings of their perianth segments. A key to the identification of the species in this genus reported in Colombia and adjacent areas is provided along with information on their ecology, habitat, and geographical ranges. A new species of Campylocentrum discovered during this study is described and the status of unconfirmed taxa briefly discussed.