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14 July 2015 Molecular variability of Chrysoperla Steinmann, 1964 (Neuroptera: Chrysopidae) inhabiting western Saudi Arabia
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Abstract

The large ribosomal RNA subunit (16S rRNA) and cytochrome C subunit 3 (CO3) genes have been partially amplified and sequenced for the species of Chrysoperla Steinmann, 1964 (Neuroptera: Chrysopidae) inhabiting the western part of Saudi Arabia to examine their molecular variability. Insect samples were collected from three distant localities, which were Tabouk in the north, Jeddah in the middle and Jazan in the south of the country. The concatenated sequences (904 bp) have been manipulated by three analytical methods (maximum-parsimony, neighbor-joining and maximum-likelihood) producing one fixed tree. The tree topology divided, with strong statistical supports, Saudi Arabian Chrysoperla into two clusters. Jeddah and Tabouk samples clustered (BP = 100%) with both Chrysoperla carnea (Stephens, 1836) and Chrysoperla nipponensis (Okamoto, 1914), while Jazan samples grouped (BP = 100%) with Dichochrysa tacta (Navás, 1921). Both pairwise genetic distances obtained from the concatenated data and amino acid substitution in CO3 gene supported this division. It could be concluded that Saudi Arabian Chrysoperla constitutes a species complex and requires further molecular investigation.

Samy M. Sayed and Sayed A. Amer "Molecular variability of Chrysoperla Steinmann, 1964 (Neuroptera: Chrysopidae) inhabiting western Saudi Arabia," The Pan-Pacific Entomologist 91(2), 101-107, (14 July 2015). https://doi.org/10.3956/2015-91.2.101
Received: 26 November 2014; Published: 14 July 2015
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