Pterostichus (Leptoferonia) hackerae sp. nov. is described based on a single male specimen with its type locality No Name Cave, Josephine Co., Oregon. This is the seventh species of microphthalmous Leptoferonia Casey, 1918 to be described, and based on the analysis of DNA data, it is found to be most closely related to another microphthalmous species, P. rothi (Hatch, 1951). A second specimen and location for P. (Leptoferonia) enyo Will, 2007, also a microphthalmous species, is reported. Analysis of DNA sequence data (28S rDNA and COI mtDNA) places P. enyo as sister to the more southern microphthalmous species P. (Leptoferonia) caligans Horn, 1891. A modified version of the key by Hacker (1968) is presented, allowing for identification of all known species of Leptoferonia.