Many Phrynosoma species exhibit blood-squirting behavior, shooting a stream of blood from the ocular area, but not all species are known to exhibit this behavior. I tested blood-squirting responses to Canis familiaris in P. ditmarsi and P. solare, and recorded incidences of this behavior in other Phrynosoma species (P. asio, P. braconnieri, P. cornutum, P. orbiculare, and P. taurus). Phrynosoma ditmarsi squirted blood in 2 of 3 trials with the dog, but no P. ditmarsi showed any sign of blood squirting while handled in the field (n = 17). No P. solare squirted blood while handled (n = 7), but 1 specimen did squirt blood during a predator trial. Phrynosoma asio and P. cornutum both squirted blood more frequently when handled and other species did not squirt blood. This study corroborates previous observations that the behavior is context specific and can be elicited by tactile stimulation. This is the first published account of the blood squirting behavior in P. ditmarsi.