This paper reports shrews (Soricidae, Soricomorpha) in Colima, Mexico, namely, Cryptotis parva, Sorex saussurei and the threatened Megasorex gigas. They were recorded in cloud, pine, and pine-oak forests. Voucher specimens were catalogued in the Colección Nacional de Mamíferos (CNMA), Instituto de Biología de la Universidad Nacional Autónoma de México (UNAM), in Mexico City. This research contributes to the knowledge of the biodiversity of western Mexico, provides the first museum specimens of these taxa in the CNMA and confirms the presence of taxa of conservation concern.