On the Llano Estacado of Texas, roads and agricultural development have divided once continuous prairie into a patchwork of habitats that differ in size and use. In an agricultural matrix, populations of small mammals were studied to assess effects of roads and corridors on movement and dispersal. During 52,416 trap nights and by tracking mammals dusted with fluorescent powder, we investigated frequency that animals crossed roads and irrigated crop circles. Sites bisected by wide roads with corridors had greater richness; whereas, sites divided by narrow roads with corridors had a significantly greater abundance of hispid cotton rats (Sigmodon hispidus). Based on our studies using powder-tracking, only reproductively active males of North American deermice (Peromyscus maniculatus) and western harvest mice (Reithrodontomys megalotis) moved significantly more along roads than away from or toward roads. Our results suggest that unfarmed crop corners are valuable habitat for wildlife; small mammals present may be impacted differentially by type of roadways that are present.