Isolation and characterization of polymorphic microsatellite DNA Markers from an Amazonian white-sand vegetation specialist bird, Xenopipo atronitens (Aves: Pipridae)

Chrysoula Gubili; Camila Duarte Ritter; Jessica Motta; Izeni P. Farias; John Bates; Roberta Canton; João M. G. Capurucho; Cintia Cornelius; Kevin A. Feldheim; Camila C. Ribas

doi:10.1676/1559-4491-128.3.668

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1 September 2016 Isolation and characterization of polymorphic microsatellite DNA Markers from an Amazonian white-sand vegetation specialist bird, Xenopipo atronitens (Aves: Pipridae)

Chrysoula Gubili, Camila Duarte Ritter, Jessica Motta, Izeni P. Farias, John Bates, Roberta Canton, João M. G. Capurucho, Cintia Cornelius, Kevin A. Feldheim, Camila C. Ribas

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The Wilson J. of Ornithology, 128(3):668-672 (2016). https://doi.org/10.1676/1559-4491-128.3.668

Abstract

Thirteen novel polymorphic microsatellite loci were isolated and characterized from the Amazonian Black Manakin, Xenopipo atronitens. The loci were screened using 30 samples from Brazil. A total of 13 loci were variable with number of alleles per locus ranging from 2 to 17, whilst the observed and expected heterozygosities varied from 0.067–0.900 and 0.310–0.910, respectively. Five loci deviated significantly from the Hardy–Weinberg equilibrium, whereas one pair of loci deviated significantly from linkage disequilibrium. The set of markers will be a useful tool for future population genetic and kinship studies of X. atronitens, an Amazonian white-sand vegetation specialist.

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Chrysoula Gubili, Camila Duarte Ritter, Jessica Motta, Izeni P. Farias, John Bates, Roberta Canton, João M. G. Capurucho, Cintia Cornelius, Kevin A. Feldheim, and Camila C. Ribas "Isolation and characterization of polymorphic microsatellite DNA Markers from an Amazonian white-sand vegetation specialist bird, Xenopipo atronitens (Aves: Pipridae)," The Wilson Journal of Ornithology 128(3), 668-672, (1 September 2016). https://doi.org/10.1676/1559-4491-128.3.668

Received: 27 May 2015; Accepted: 1 December 2015; Published: 1 September 2016

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