The scope of this study is to evaluate the faunistic, ecological and zoogeographical composition of the subfamily Steninae (Coleoptera: Staphylinidae) of the Central Anatolian Region of Turkey. Thirty-eight species, belonging to two genera, were detected by field studies between 2009-2011 in the Central Anatolian Region. It is concluded that among these, 12 species represent a first record for Central Anatolian Region and one species (Stenus distortus Assing, 2006) is endemic for Turkey. In previous studies, five species belonging to Stenus were recorded from Turkey, however, it was not clear that which provinces they were collected from. With this study, detailed localities are provided (Stenus ater Mannerheim, 1830, S. atratulus Erichson, 1839, S. carbonarius Gyllenhal, 1827, S. crassus Stephens, 1833, S. formicetorum Mannerheim, 1843).