This paper presents an analysis of tree-ring growth patterns of male and female Fraxinus mandshurica trees from 1931 to 2007. The specific object was to study the response of radial growth to climate variables separately for male and female trees. The results show that the growth patterns in the two genders were similar during the mid-1950s to 1970s but different in the periods 1931–1940s and 1980–2007. In the period 1980–2007, the mean sensitivity and mean widths of the tree rings were significantly different between the genders (p < 0.05). The climate-growth response in female and male trees was also different. Female trees are sensitive to precipitation in November of the previous year, whereas male trees respond to mean temperature in November of the previous year. The results confirm that climatic sensitivity in male and female trees of dioecious species is different, yet this difference is not stable through time.