Barcoding DNA is an accepted tool in taxonomic identification. We report the design of a universal primer pair for polymerase chain reaction (PCR) amplification of the cytochrome c oxidase subunit I gene (COI) developed using COI gene sequences of all 8 extant bear species (family Ursidae) available on GenBank. This primer pair successfully amplified approximately 700 base pairs of the COI gene of Asiatic black (Ursus thibetanus; n = 12) and Himalayan brown (U. arctos; n = 6) bears. We sequenced the PCR product and compared the sequences with those of the other 6 species to generate degrees of homology (83–92%) and genetic distances. The primer pair has yet to be tested in the other 6 species. We developed a preliminary phylogenetic tree for the 8 species based on these data.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 25 • No. 1