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1 January 2013 A Trp574 to Leu Amino Acid Substitution in the ALS Gene of Annual Bluegrass (Poa annua) Is Associated with Resistance to ALS-Inhibiting Herbicides
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Abstract

Annual bluegrass is commonly controlled by acetolactate synthase (ALS)-inhibiting herbicides in managed turfgrass. An annual bluegrass population with suspected resistance to ALS-inhibiting herbicides was collected from Grand National Golf Course in Opelika, AL (GN population). Subsequent testing confirmed resistance of the GN population to foramsulfuron, trifloxysulfuron, bispyribac-sodium (bispyribac), and imazaquin when compared to a susceptible population collected locally at Auburn University (AU population). Sequencing of the ALS gene revealed a point mutation resulting in an amino acid substitution at Trp574. Cloning of the ALS gene surrounding the Trp574 region yielded two distinct ALS gene sequences: one producing Trp574 and one producing Leu574. Trp574 to Leu has been previously correlated with resistance to ALS-inhibiting herbicides. Both AU and GN gene sequences contained other similar silent and missense mutations. This research confirms resistance of annual bluegrass to ALS-inhibiting herbicides with Trp574 to Leu amino acid substitution being the most likely mode of resistance based on past literature.

Nomenclature: Bispyribac; foramsulfuron; imazaquin; trifloxysulfuron; annual bluegrass, Poa annua L.

Weed Science Society of America
J. Scott McElroy, Michael L. Flessner, Zhuoyu Wang, Fenny Dane, Robert H. Walker, and Glenn R. Wehtje "A Trp574 to Leu Amino Acid Substitution in the ALS Gene of Annual Bluegrass (Poa annua) Is Associated with Resistance to ALS-Inhibiting Herbicides," Weed Science 61(1), 21-25, (1 January 2013). https://doi.org/10.1614/WS-D-12-00068.1
Received: 4 May 2012; Accepted: 4 July 2012; Published: 1 January 2013
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