Translator Disclaimer
1 May 2017 Multiple Resistance to Glyphosate and Acetolactate Synthase Inhibitors in Palmer Amaranth (Amaranthus palmeri) Identified in Brazil
Anita Küpper, Ednaldo A. Borgato, Eric L. Patterson, Acácio Gonçalves Netto, Marcelo Nicolai, Saul J. P. de Carvalho, Scott J. Nissen, Todd A. Gaines, Pedro J. Christoffoleti
Author Affiliations +
Abstract

Palmer amaranth is native to the United States, but was discovered in 2015 in Brazil. Palmer amaranth populations in Brazil were very difficult to control using glyphosate, which resulted in many changes to standard weed management practices. A genotyping assay was used to confirm that the population detected in Mato Grosso State, Brazil, was correctly identified as Palmer amaranth and that it was not tall waterhemp. Greenhouse dose—response curves and shikimate accumulation assays showed that the Brazilian population was highly resistant to glyphosate, with an LD50 value (3,982 g glyphosate ha-1) more than twice the typical use rates and very little shikimate accumulation at 1 mM glyphosate concentrations in a leaf-disk assay. The Brazilian population was also resistant to sulfonylurea and imidazolinone acetolactate synthase (ALS) inhibitor herbicides. The resistance mechanisms in the Brazilian population were identified as increased EPSPS gene copy number for glyphosate resistance (between 50- and 179-fold relative EPSPS gene copy number increase) and two different alleles for target-site mutations in the ALS gene (W574L and S653N). These results confirm the introduction of Palmer amaranth to Brazil using a genetic marker for species identification, as well as resistance to glyphosate and ALS inhibitors.

Nomenclature: Glyphosate; Palmer amaranth, Amaranthus palmeri S. Wats; tall waterhemp, Amaranthus tuberculatus (Moq.) Sauer.

© Weed Science Society of America, 2017
Anita Küpper, Ednaldo A. Borgato, Eric L. Patterson, Acácio Gonçalves Netto, Marcelo Nicolai, Saul J. P. de Carvalho, Scott J. Nissen, Todd A. Gaines, and Pedro J. Christoffoleti "Multiple Resistance to Glyphosate and Acetolactate Synthase Inhibitors in Palmer Amaranth (Amaranthus palmeri) Identified in Brazil," Weed Science 65(3), 317-326, (1 May 2017). https://doi.org/10.1017/wsc.2017.1
Received: 28 September 2016; Accepted: 31 December 2016; Published: 1 May 2017
JOURNAL ARTICLE
10 PAGES


Share
SHARE
KEYWORDS
acetolactate synthase
chlorsulfuron
EPSPS
EPSPS copy number
imazethapyr
multiple resistance
sulfometuron
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top