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1 March 2018 Target-Site Point Mutation Conferring Resistance to Trifluralin in Rigid Ryegrass (Lolium rigidum)
Benjamin Fleet, Jenna Malone, Christopher Preston, Gurjeet Gill
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Abstract

Populations of rigid ryegrass suspected of resistance to trifluralin due to control failures exhibited varying levels of susceptibility to trifluralin, with 15 out of 17 populations deemed resistant (>20% plant survival). Detailed dose-response studies were conducted on one highly resistant field-evolved population (SLR74), one known multiply resistant population (SLR31), and one susceptible population (VLR1). On the basis of the dose required to kill 50% of treated plants (LD50), SLR74 had 15-fold greater resistance than VLR1, whereas, the multiply resistant SLR31 had 10-fold greater resistance than VLR1. Similarly, on the basis of dose required to reduce shoot biomass by 50% (GR50), SLR74 had 17-fold greater resistance than VLR1, and SLR31 had 8-fold greater resistance than VLR1. Sequencing of the α-tubulin gene from resistant plants of different populations confirmed the presence of a previously known goosegrass mutation causing an amino acid substitution at position 239 from threonine to isoleucine in resistant population SLR74. This mutation was also found in 4 out of 5 individuals in another highly resistant population TR2 and in 3 out of 5 individuals of TR4. An amino acid substitution from valine to phenylalanine at position 202 was also observed in TR4 (3 out of 5 plants) and TR2 (1 out of 5 plants). There was no target-site mutation identified in SLR31. This study documents the first known case of field-evolved target-site resistance to dinitroaniline herbicides in a population of rigid ryegrass.

Nomenclature: Trifluralin; rigid ryegrass; Lolium rigidum Gaudin; goosegrass; Eleusine indica (L.) Gaertn.

© Weed Science Society of America, 2017
Benjamin Fleet, Jenna Malone, Christopher Preston, and Gurjeet Gill "Target-Site Point Mutation Conferring Resistance to Trifluralin in Rigid Ryegrass (Lolium rigidum)," Weed Science 66(2), 246-253, (1 March 2018). https://doi.org/10.1017/wsc.2017.67
Received: 26 July 2017; Accepted: 1 September 2017; Published: 1 March 2018
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KEYWORDS
dinitroaniline herbicides
Herbicide resistance
mechanism
target-site
α-Tubulin mutation
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