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1 January 2019 Proline-106 EPSPS Mutation Imparting Glyphosate Resistance in Goosegrass (Eleusine indica) Emerges in South America
Hudson K. Takano, Rafael R. Mendes, Leonardo B. Scoz, Ramiro F. Lopez Ovejero, Jamil Constantin, Todd A. Gaines, Philip Westra, Franck E. Dayan, Rubem S. Oliveira
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Abstract

Glyphosate-resistant (GR) goosegrass [Eleusine indica (L.) Gaertn.] was recently identified in Brazil, but its resistance mechanism was unknown. This study elucidated the resistance mechanism in this species and developed a molecular marker for rapid detection of this target-site resistance trait. The resistance factor for the resistant biotype was 4.4-fold compared with the glyphosate-susceptible (GS) in greenhouse dose–response experiments. This was accompanied by a similar (4-fold) difference in the levels of in vitro and in planta shikimate accumulation in these biotypes. However, there was no difference in uptake, translocation, or metabolism of glyphosate between the GS and GR biotypes. Moreover, both biotypes showed similar values for 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) copy number and transcription. Sequencing of a 330-bp fragment of the EPSPS gene identified a single-nucleotide polymorphism that led to a Pro-106-Ser amino acid substitution in the enzyme from the GR biotype. This mutation imparted a 3.8-fold increase in the amount of glyphosate required to inhibit 50% of EPSPS activity, confirming the role of this amino acid substitution in resistance to glyphosate. A quantitative PCR–based genotyping assay was developed for the rapid detection of resistant plants containing this Pro-106-Ser mutation.

© Weed Science Society of America, 2018.
Hudson K. Takano, Rafael R. Mendes, Leonardo B. Scoz, Ramiro F. Lopez Ovejero, Jamil Constantin, Todd A. Gaines, Philip Westra, Franck E. Dayan, and Rubem S. Oliveira "Proline-106 EPSPS Mutation Imparting Glyphosate Resistance in Goosegrass (Eleusine indica) Emerges in South America," Weed Science 67(1), 48-56, (1 January 2019). https://doi.org/10.1017/wsc.2018.71
Received: 24 May 2018; Accepted: 26 August 2018; Published: 1 January 2019
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KEYWORDS
5-enolpyruvylshikimate-3-phosphate synthase
Absorption and translocation
EPSPS
glyphosate metabolism
herbicideresistance detection, shikimate accumulation
target-site resistance
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