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29 October 2019 First Report of Molecular Basis of Resistance to Imazethapyr in Common Lambsquarters (Chenopodium album)
Zhaofeng Huang, Xinxin Zhou, Chaoxian Zhang, Cuilan Jiang, Hongjuan Huang, Shouhui Wei
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Abstract

Common lambsquarters (Chenopodium album L.) is one of the most troublesome weeds in soybean [Glycine max (L.) Merr.] and corn (Zea mays L.) fields in northeast China. In 2017, a C. album population that survived imazethapyr at the recommended field rate was collected from a soybean field in Heilongjiang Province in China. Experiments were conducted to determine the basis of resistance to imazethapyr and investigate the herbicide-resistance pattern in C. album. Dose–response tests showed that the resistant population (R) displayed high resistance to imazethapyr (20-fold) compared with the susceptible population (S). An in vitro acetolactate synthase (ALS) activity assay indicated that the ALS of the R population was resistant to imazethapyr compared with the ALS of the S population. Sequence analysis of the ALS gene revealed that the GCA was replaced by ACA at amino acid position 122, which resulted in an alanine to threonine substitution (Ala-122-Thr) in the R population. The R population displayed cross-resistance to thifensulfuron-methyl and flumetsulam but susceptibility to bispyribac-sodium, flucarbazone, glyphosate, mesotrione, and fomesafen. These results confirmed that the basis of imazethapyr resistance in C. album was conferred by the Ala-122-Thr substitution in the ALS enzyme. This is the first report of the target-site basis of ALS-inhibiting herbicide resistance in C. album.

© Weed Science Society of America, 2019.
Zhaofeng Huang, Xinxin Zhou, Chaoxian Zhang, Cuilan Jiang, Hongjuan Huang, and Shouhui Wei "First Report of Molecular Basis of Resistance to Imazethapyr in Common Lambsquarters (Chenopodium album)," Weed Science 68(1), 63-68, (29 October 2019). https://doi.org/10.1017/wsc.2019.62
Received: 2 August 2019; Accepted: 20 October 2019; Published: 29 October 2019
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