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1 October 2001 Confirmation of an Enzyme-Linked Immunosorbent Assay to Detect Fluometuron in Soil
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Research was conducted to compare the results of an enzyme-linked immunosorbent assay (ELISA) to high-performance liquid chromatography (HPLC) for detecting fluometuron in the environment. A linear relationship for HPLC (R2 > 0.90) and ELISA (R2 > 0.66) analysis was observed between the natural logarithm of the detected fluometuron concentrations regressed against time in soil collected from a cropped area, a grass filter strip, and a riparian forest. Both methods detected the same initial fluometuron concentration (y-intercept) for two of the three soils evaluated. The ELISA and HPLC measurements of fluometuron concentrations compared favorably with r values from 0.83 to 0.98. Predicted fluometuron half-lives determined from HPLC and ELISA measurements were: 110 and 112 d in the cropped watershed, 28 and 29 d in the riparian area, and 11 and 11 d in the grass filter strip, respectively. Results from both techniques indicated shorter half-lives in soil from the grass filter strip and riparian area than in cropped area soil. There was an inverse correlation between predicted half-lives and soil organic matter, pH, clay, and cation exchange capacity.

Additional index word: Herbicide degradation.

Abbreviations: BMPs, best management practices; CEC, cation-exchange capacity; DAT, days after treatment; DT50, 50% disappearance time; ELISA, enzyme-linked immunosorbent assay; HPLC, high-performance liquid chromatography; IC50, analyte concentration that decreases chromogen activity by 50%; LDD, least detectable dose; MD-MSEA, Mississippi Delta Management Systems Evaluation Area; OM, organic matter.

MARK W. SHANKLE, DAVID R. SHAW, and MICHELE BOYETTE "Confirmation of an Enzyme-Linked Immunosorbent Assay to Detect Fluometuron in Soil," Weed Technology 15(4), 669-675, (1 October 2001).[0669:COAELI]2.0.CO;2
Published: 1 October 2001
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