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1 January 2017 Glyphosate-Resistant Palmer Amaranth (Amaranthus palmeri) in Nebraska: Confirmation, EPSPS Gene Amplification, and Response to POST Corn and Soybean Herbicides
Parminder S. Chahal, Vijay K. Varanasi, Mithila Jugulam, Amit J. Jhala
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Abstract

Palmer amaranth is the most problematic weed in agronomic crop production fields in the United States. A Palmer amaranth biotype was not controlled with sequential applications of glyphosate in glyphosate-resistant (GR) soybean production field in south-central Nebraska. The seeds of the putative GR Palmer amaranth biotype were collected in the fall of 2015. The objectives of this study were to (1) confirm GR Palmer amaranth and determine the level of resistance in a whole-plant dose-response bioassay, (2) determine the copy number of 5-enolpyruvylshikimate-3-phosphate (EPSPS) gene, the molecular target of glyphosate, and (3) evaluate the response of GR Palmer amaranth biotype to POST corn and soybean herbicides with different modes-of-action. Based on the effective dose required to control 90% of plants (ED90), the putative GR Palmer amaranth biotype was 37- to 40-fold resistant to glyphosate depending on the glyphosate-susceptible (GS) used as a baseline population. EPSPS gene amplification was present in the GR Palmer amaranth biotype with up to 32 to 105 EPSPS copies compared to the known GS biotypes. Response of GR Palmer amaranth to POST corn and soybean herbicides suggest reduced sensitivity to atrazine, hydroxyphenylpyruvate dioxygenase (HPPD)- (mesotrione, tembotrione, and topramezone), acetolactate synthase (ALS)- (halosulfuron-methyl), and protoporphyrinogen oxidase (PPO)- (carfentrazone and lactofen) inhibitors. GR Palmer amaranth was effectively controlled (>90%) with glufosinate applied at 593 g ai ha−1 with ≥95% reduction in biomass. More research is needed to determine whether this biotype exhibits multiple resistant to other group of herbicides and evaluate herbicide programs for effective management in corn and soybean.

Nomenclature: 2,4-D; acetochlor; acifluorfen; atrazine; bentazon; bromoxynil; carfentrazone; chlorimuron; dicamba; fluthiacet; fomesafen; glufosinate; glyphosate; halosulfuron; imazamox; imazethapyr; lactofen; mesotrione; S-metolachlor; tembotrione; thiencarbazone; thifensulfuron; topramezone; Palmer amaranth, Amaranthus palmeri S. Wats.; corn, Zea mays L.; soybean, Glycine max (L.) Merr.

Amaranthus palmeri es la malezas más problemática en campos de producción de cultivos agronómicos en los Estados Unidos. Un biotipo de A. palmeri no fue controlado con aplicaciones secuenciales de glyphosate en un campo de producción de soja resistente a glyphosate (GR) en el sur central de Nebraska. Las semillas del biotipo putativo GR de A. palmeri fueron colectadas en el otoño de 2015. Los objetivos de este estudio fueron (1) confirmar que A. palmeri es GR y determinar el nivel de resistencia en un bioensayo de respuesta a dosis con plantas completas, (2) determinar el número de copias del gen 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), el objetivo molecular de glyphosate, y (3) evaluar la respuesta del biotipo GR de A. palmeri a herbicidas POST para maíz y soja con diferentes modos de acción. Con base en la dosis efectiva requerida para controlar 90% de las plantas (ED90), el biotipo putativo GR de A. palmeri fue 37 a 40 veces más resistente a glyphosate dependiendo de la población susceptible a glyphosate (GS) base utilizada. La amplificación del gen EPSPS estuvo presente en el biotipo GR de A. palmeri con 32 y hasta 105 copias más de EPSPS comparado con biotipos GS conocidos. La respuesta de A. palmeri GR a herbicidas POST para maíz y soja sugiere una sensibilidad reducida a atrazine, y a inhibidores de hydroxy phenylpyruvate dioxygenase (HPPD) (mesotrione, tembotrione, y topramezone), de acetolactate synthase (ALS) (halosulfuron-methyl), y de protoporphyrinogen oxidase (PPO) (carfentrazone y lactofen). A. palmeri GR fue efectivamente controlado (>90%) con glufosinate aplicado a 59

© Weed Science Society of America, 2017 
Parminder S. Chahal, Vijay K. Varanasi, Mithila Jugulam, and Amit J. Jhala "Glyphosate-Resistant Palmer Amaranth (Amaranthus palmeri) in Nebraska: Confirmation, EPSPS Gene Amplification, and Response to POST Corn and Soybean Herbicides," Weed Technology 31(1), 80-93, (1 January 2017). https://doi.org/10.1614/WT-D-16-00109.1
Received: 22 July 2016; Accepted: 9 September 2016; Published: 1 January 2017
KEYWORDS
EPSPS gene copy number
glyphosate-susceptible
herbicide efficacy
resistance confirmation
resistance management
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