The determination of the anisotropic and heterogeneous character of the saturated hydraulic conductivity (K_s) of wetland soils is technically challenging, but is crucial for the accurate quantification of flow and transport processes. We modified a laboratory method to determine K_s both in the vertical (K_s,v) and horizontal directions (K_s,h), and tested it on the same undisturbed peat samples using a constant head upward flow permeameter. The first results showed that K_s,v was greater than K_s,h in the majority of samples from two profiles of a degraded fen peat, indicating that K_s was anisotropic. In conclusion, the described method was suitable to determine K_s,v and K_s,h and can be recommended to estimate the anisotropy of K_s in wetland soils.