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12 December 2017 Variation in Major Histocompatibility Complex diversity in invasive cane toad populations
Mette Lillie, Sylvain Dubey, Richard Shine, Katherine Belov
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Abstract

Context. The cane toad (Rhinella marina), a native species of central and southern America, was introduced to Australia in 1935 as a biocontrol agent after a complex history of prior introductions. The population rapidly expanded and has since spread through much of the Australian landmass, with severe impacts on the endemic wildlife, primarily via toxicity to predators. The invasion process has taken its toll on the cane toad, with changes in the immunological capacity across the Australian invasive population.

Aims. To investigate the immunogenetic underpinnings of these changes, we studied the diversity of the Major Histocompatiblity Complex (MHC) genes in introduced cane toad populations.

Methods. We studied the diversity of two MHC genes (the classical class I UA locus and a class II DAB locus) and compared these with neutral microsatellite markers in toads from the Australian site of introduction and the Australian invasion front. We also included toads from Hawai’i, the original source of the Australian toads, to infer founder effect.

Key results. Diversity across all markers was low across Australian and Hawai’ian samples, consistent with a reduction in genetic diversity through multiple founder effects during the course of the successive translocations. In Australia, allelic diversity at the microsatellite markers and the UA locus was reduced at the invasion front, whereas all three alleles at the DAB locus were maintained in the invasion-front toads.

Conclusions. Loss of allelic diversity observed at the microsatellite markers and the UA locus could be the result of drift and bottlenecking along the invasion process, however, the persistence of DAB diversity warrants further investigation to disentangle the evolutionary forces influencing this locus.

Implications. Through the use of different molecular markers, we provide a preliminary description of the adaptive genetic processes occurring in this invasive population. The extremely limited MHC diversity may represent low immunogenetic competence across the Australian population, which could be exploited for invasive species management.

© CSIRO 2017
Mette Lillie, Sylvain Dubey, Richard Shine, and Katherine Belov "Variation in Major Histocompatibility Complex diversity in invasive cane toad populations," Wildlife Research 44(6–7), 565-572, (12 December 2017). https://doi.org/10.1071/WR17055
Received: 2 December 2016; Accepted: 12 September 2017; Published: 12 December 2017
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KEYWORDS
Bufo marinus
GENETIC DRIFT
MHC
microsatellite markers
range expansion
ARTICLE IMPACT
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