Context. As urban landscapes proliferate globally, the need for research into urban wildlife interactions is magnified. The eastern grey kangaroo (Macropus giganteus) is a widespread species commonly involved in wildlife–vehicle collisions in urban areas in Australia. Despite the many urban kangaroo populations and associated conflicts with human activities, few studies have examined how eastern grey kangaroos interact with, and are affected by, the urban matrix.

Aims. The present study aimed to quantify kangaroo demography, movements, habitat utilisation and exposure to risks during a period of intensive urban development in a rapidly changing suburb located in a region undergoing high urban growth rates.

Methods. We utilised foot-based census surveys, global positioning system (GPS) collars, direct observations and reports of wildlife mortality between 2014 and 2016. Geographical information systems (GIS) were used to integrate GPS-tracking data with spatial layers, to quantify kangaroo movements and habitat utilisation.

Key results. The kangaroo population underwent a steep decline and kangaroo–vehicle collisions were the main source of mortality (73%) during the study period. Kangaroos were regularly exposed to the risk of injury, with roads intersecting many parts of their home range. Kangaroos showed positive habitat selection both for lawn and forest habitats and kangaroo movement and presence at the study site were influenced by high-quality forage and cover.

Conclusions. The present research has highlighted that despite areas of suitable habitat remaining, road-kill was a major contributor to localised kangaroo-population decline. We showed that habitat preferences of eastern grey kangaroos in this urban area were consistent with those in natural landscapes.

Implications. The present study is the first to implicate kangaroo–vehicle collisions as the major factor in population decline in kangaroos. These findings can be utilised to guide design and placement of kangaroo–vehicle collision mitigation and assist in planning of urban areas, particularly where kangaroo populations are in decline. Local extirpation of urban kangaroo populations would be greatly reduced by incorporating site-specific kangaroo habitat preferences and existing patterns of kangaroo habitat use in infrastructure planning. The study has contributed to our understanding of the effects of roads on urban wildlife in general and highlighted the importance of landscape permeability.