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1 June 1997 Characterization of Protease-Releasing Factors Isolated from Hemocytes of the Solitary Ascidian, Halocynthia roretzi
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Abstract
We have previously demonstrated that hemocytes of the solitary ascidian Halocynthia roretzi respond to several stimuli, such as calcium ionophore, lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA), and to release metalloproteases. Here, we show that H. roretzi hemocytes contained two substances, named protease-releasing factors A and B, which induced the release of metalloproteases from the hemocytes. Factor A was isolated from the acid-ethanol extract of hemocytes by gel filtration, while factor B was isolated from the hypotonic extract of hemocytes by cation exchange chromatography. The former factor was a heat-labile, large molecule and the latter was a heat-stable, small molecule. We found that these factors existed only in some types of hemocytes.
Kaoru Azumi and Hideyoshi Yokosawa "Characterization of Protease-Releasing Factors Isolated from Hemocytes of the Solitary Ascidian, Halocynthia roretzi," Zoological Science 14(3), (1 June 1997). https://doi.org/10.2108/zsj.14.391
Received: 22 January 1997; Accepted: 1 February 1997; Published: 1 June 1997
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