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1 November 2002 Cultivation and Characterization of Planarian Neuronal Cells Isolated by Fluorescence Activated Cell Sorting (FACS)
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Abstract

Studies using molecular markers have revealed that planarians possess a highly organized brain. Here we separated brain neurons from dissociated planarian head cells by fluorescence activated cell sorting (FACS), and characterized them by single cell PCR analysis and cell culture. Dissociated cells were labeled with three different fluorescent dyes, Hoechst 33258, Merocyanine 540, and Propidium Iodide (PI), and fractioned by FACS. Interestingly, we have succeeded in identifying a cell fraction specific to the head, which we have named the head-abundant cell fraction (HAC). Most of the HAC expressed neuron-specific genes and proteins. When they were cultured in vitro, they showed an ability to extend neurites on several types of extracellular matrices (ECMs), and, depending on the ECM type used, presented a high level of plasticity in morphology and gene expression.

Maki Asami, Tsuyoshi Nakatsuka, Tetsutaro Hayashi, Kenji Kou, Hiroaki Kagawa, and Kiyokazu Agata "Cultivation and Characterization of Planarian Neuronal Cells Isolated by Fluorescence Activated Cell Sorting (FACS)," Zoological Science 19(11), 1257-1265, (1 November 2002). https://doi.org/10.2108/zsj.19.1257
Received: 29 August 2002; Accepted: 1 September 2002; Published: 1 November 2002
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