Open Access
How to translate text using browser tools
27 January 2004 The Changes of Gene Expression in Honeybee (Apis mellifera) Brains Associated with Ages
Mayumi Tsuchimoto, Makoto Aoki, Mamoru Takada, Yoshinori Kanou, Hiromi Sasagawa, Yasuo Kitagawa, Tatsuhiko Kadowaki
Author Affiliations +

Honeybee (Apis mellifera) worker bees (workers) are known to perform wide variety of tasks depending on their ages. The worker's brains also show the activity and behavior-dependent chemical and structural plasticity. To test if there are any changes of gene expression associated with different ages in the worker brains, we compared the gene expression patterns between the brains of newly emerged bees and old foraging workers (foragers) by macroarray analysis. The expression of genes encoding signal transduction pathway components, ion channels, and neurotransmitter transporters is elevated in the old forager brains, suggesting that the neuronal activities would be enhanced. The mRNA levels of cell adhesion protein, transcription related factors, and plasma membrane associated proteins are also increased in the old forager brains. Meanwhile, the mRNA level of one putative cell adhesion protein is decreased in the old forager brains. These results thus suggest that the dramatic changes of gene expression occur in honeybee brains associated with ages.


Honeybee, Apis mellifera, is one of social insects and has been used as a model system to study complex animal behavior in addition to learning and memory. Honeybees form a colony, which is consisted of single queen, hundreds of male drone, and thousands of female worker bees. In contrast to a queen and male bees, workers perform wide variety of tasks to maintain the colony. Young workers feed and care for larvae and queen, middle-age workers maintain the hive and store food, and old workers forage for nectar and pollen outside the hive (age-related division of labor). In contrast to performing tasks inside the dark hive, foraging involves extensive flight, learning and memorizing food locations, navigation using multiple cues (for example, sun compass), and communication by a dance language (Fahrbach and Robinson, 1995; Menzel and Muller, 1996; Menzel and Giurfa, 2001). These tasks may require better modules for sensory-input processing and enhanced learning and memory capabilities. This behavioral shift is known to accompany changes in endocrine system, brain chemistry, and brain structure. The levels of juvenile hormone and biogenic amines (for example, octopamine) in the brain are higher in old forager compared with young in-hive worker (Fahrbach and Robinson, 1996; Wagener-Hulme et al., 1999). The volumes of certain glomerulus in antennal lobe and the neuropils of mushroom bodies also increase depending on the age and activity (Sigg et al., 1997; Withers et al., 1993). Moreover, it has been recently shown that mushroom body intrinsic neurons of forager brain have longer and more branched dendrites than those of in-hive bee brain (Farris et al., 2001).

It is not known whether the long-term changes in neuronal function, which underlie the age-related division of labor under natural condition are dependent on the changes of gene expression. In fact, there are a few studies reporting the changes of gene expression in the brain that are associated with behavioral plasticity. The expression of ZENK (one of immediate early genes) and the genes encoding glucocorticoid receptor, serotonin receptor, and nerve growth factor is induced in hippocampus by environmental enrichment in adult rats (Olsson et al., 1994; Rasmuson et al., 1998). In songbirds, the expression of sex steroid receptors and SNAg (song system nuclear antigen) in song behavior control region of brain is modulated by sex, age, and season (Gahr and Metzdorf, 1997; Akutagawa and Konishi, 2001). In honeybees, the levels of period and PKG (cGMP dependent protein kinase) mRNAs are higher in the foragers than the in-hive bees (Toma et al., 2000; Ben-Shahar et al., 2002).

To unveil the changes of gene expression in honeybee brains associated with ages, we analyzed the gene expression patterns in the brains of the workers with different ages. The relationship between the changes of gene expression in honeybee brains and the modifications of brain structures and functions associated with ages and behavioral plasticity is discussed.


Construction of honeybee brain specific cDNA library by suppression subtractive hybridization (SSH)

PolyA+RNA was isolated from the brains, hypopharyngeal glands, and abdomens of twenty foragers with a QuickPrep Micro mRNA Purification Kit (Amersham) and used for the preparation of double strand cDNAs. Brain cDNA was used as “tester” and the mixture of hypopharyngeal gland and abdomen cDNAs was used as “driver”. The SSH followed by PCR amplification was carried out according to manufacturer's instructions (CLONTECH PCR-SelectTM cDNA Subtraction Kit). The PCR amplified DNA was size-fractionated into three pools by agarose gel electrophoresis. The DNA in each pool was cloned in a pGEM-T Easy vector (Promega) by T/A cloning.

Macroarray analysis and cDNA sequencing

The cDNA insert of each recombinant plasmid was amplified by colony PCR with T7 and SP6 promoter primers. The amplification of total 1235 cDNAs was verified by agarose gel electrophoresis. The aliquot (2 μg) of the amplified DNA was denatured by heat and alkaline treatment, and then spotted to nylon membranes by a 96 wells dot blot apparatus. EF-1 α cDNA was also simultaneously spotted along with other 95 cDNA clones. PolyA+RNA was isolated from the brains of the twenty foragers and the newly emerged workers (within 24 hr after eclosion). First strand 32P-labeled cDNA probes were prepared with each polyA+RNA using 32P-dCTP, oligo dT primer, and reverse transcriptase. The two resulting probes (with equal radioactivity) were used for the hybridization of the nylon membranes prepared as above. The radioactivity of each round DNA spot was measured by a Bioimage analyzer BAS2000 (Fuji). The background correction was made by subtracting the radioactivity of the adjacent area without spotted DNA. We then calculated the ratio of the radioactivity of forager to newly emerged bee (F/NE) for each cDNA. These values were multiplied by the newly emerged to forager bee (NE/F) ratio of the radioactivity of EF-1α cDNA for the normalization of hybridization. The level of EF-1 α mRNA was previously shown to be constant during the adult honeybee development (Toma et al., 2000). These experiments were repeated three times with probes prepared by polyA+RNA isolated from different bee samples. We sequenced the differentially expressed cDNA clones with an ABI PRISM BigDye Terminator Cycle Sequencing Ready Reaction Kit and ABI PRISM 310 Genetic Analyzer (Applied Biosystems). The putative protein encoded by each cDNA was determined using a Blast-X program (Altschul et al., 1997).

Northern blot hybridization

PolyA+RNA was isolated from the brains of the twenty newly emerged workers, 7 days old in-hive workers, and 28 days old pollen foragers. 5 μg of polyA+RNA was separated by 1% agarose gel containing formaldehyde and transferred to nylon membranes. They were hybridized with various cDNA probes as indicated. These experiments were repeated three times with polyA+RNA isolated from different bee samples. The radioactivity of each band was measured by the BAS2000 and honeybee EF-1 α mRNA was used as a control to normalize the levels of loaded RNA.


We compared the gene expression patterns in the brains of newly emerged bees and unknown aged foragers by Macroarray analysis. The level of the expression of each mRNA in brain is varied. Many clones show variable expression levels between the forager and newly emerged bee brains. We sequenced the cDNA clones, which were differentially expressed between forager and newly emerged bee brains. The cDNA sequences were analyzed by a Blast-X program to search the homolog of protein encoded by each cDNA. The putative proteins coded by the cDNAs are classified by their functions and all data are summarized in Table 1. As expected, most of honeybee genes have homologs in a fruit fly, Drosophila melanogaster. Meanwhile, when they have homologs in several different species, some show higher homology to, for example, human than fruit fly genes.

Table 1

Summary of genes differentially expressed between newly emerged and forager bee brains.


All genes categorized as neurotransmitter transporters and ion channels are expressed at higher levels in the old forager compared to newly emerged bee brains. These include choline transporter (Okuda et al., 2000), glutamate transporter (Besson, et al., 1999), betaine/GABA transporter (Borden et al., 1995), cationic amino acid transporter (Hoshide et al., 1996), voltage-gated calcium channel subunit (Klugbauer et al., 1999), Na+/K+-transporting ATPase α subunit (Sun et al., 1998), and Na+-driven anion exchanger (with Na+-dependent Cl-HCO3 exchange activity) (Romero et al., 2000) genes. These results may suggest that the uptake of various neurotransmitters and the exchange of various ions through the plasma membrane in neurons and glia would be more enhanced in the old forager than newly emerged bee brains.

All genes categorized as signal transduction pathway components are expressed at higher levels in the old forager compared to newly emerged bee brains. These include phosphoinositide-specific phospholipase C (PI-PLC) epsilon (Song et al., 2001) and diacylglycerol kinase (DGK) (Harden et al., 1993) genes. These results suggest that the signal transduction mediated by the intracellular Ca2+ and phospholipid second messengers would be more intense in the old forager than newly emerged bee brains. The other genes expressed at higher levels in the old forager brains are orphan receptor tyrosine kinase (RTK) homologous to mRor1 (Oishi et al., 1999), protein kinase homologous to Drosophila CG8789, and protein tyrosine phosphatase (PTP) homologous to human PTP sigma (Krueger et al., 1990). Their functions in the adult brains are not yet identified in any species. Nevertheless, the cell signaling activities mediated by these protein kinases and phosphatase may be enhanced in the old forager compared to newly emerged bee brains. We identify the honeybee gene encoding the homolog of Drosophila soluble guanylyl cyclase β subunit, that catalyzes the synthesis of intracellular cGMP (Shah and Hyde, 1995). The resulting cGMP directly or indirectly activates PKG, cGMP-regulated phosphodiesterase, PKA, and cyclic nucleotide-gated channels. These cGMP dependent intracellular events would be more facilitated in the old forager than newly emerged bee brains. We also find the honeybee gene homologous to Drosophila still life (sif). The sif encodes a guanine nucleotide exchange factor for RAC, a member of RHO family small G proteins. SIF was shown to be necessary for the formation or maintenance of synapses (Sone et al., 2000). The higher expression of sif mRNA in the old forager brains may demonstrate that new synaptic formation or the reorganization of preexisting synapses is activated.

We identify two honeybee genes encoding the homologs of cell adhesion proteins, Drosophila Sidekick (SDK) and GH11322 in this screen. Expression of the former gene but not the latter is enhanced in the old forager brains. It is intriguing that the expression of the GH11322 homologous gene is in fact reduced in the old forager brains (see Fig. 1). Drosophila SDK appears to be necessary for the correct pattern formation of photoreceptor cells (Nguyen et al., 1997). Thus, new synaptic formation or the modifications of synaptic terminals in visual system may occur more frequently in the old foragers. Drosophila GH11322 cDNA encodes a protein with four immunoglobulin domains, two fibronectin type III domains, and one transmembrane domain. In Drosophila, this gene lies next to turtle (tutl), which has an essential role for coordinated motor output. The phenotypes of the disruption of both tutl and GH11322 genes are identical to those of the single disruption of tutl, suggesting that their functions are not redundant (Bodily et al., 2001). Because these two proteins have similar extracellular but different intracellular domains, they can bind the same targets but result in the different outcomes. The functions of GH11322 in the brain remain to be established.

Fig. 1

The relative levels of betaine/GABA transporter, Na+/K+-transporting ATPase α subunit, PI-PLC epsilon, orphan RTK, and Drosophila GH11322 homolog mRNAs in the brains of bees at 0 (solid bars; normalized as 1), 7 (striped bars), and 28 (hatched bars) days after eclosion. The 7 and 28 days old bees are the in-hive and pollen forager bees, respectively. These values are means±SEM of three independent experiments.


We find that two honeybee genes encoding transcription related factors are expressed at higher levels in the old forager brains. These are one of transcription mediator complex subunits (Trap36) (Boube et al., 2000) and transcription repressor for particular genes (Sin3A) (Neufeld et al., 1998). These results may suggest that the stimulation and repression of the transcription of specific genes are more tightly regulated in the old forager compared to newly emerged bee brains.

All genes categorized as other functional protein genes are expressed at higher levels in the old forager compared to newly emerged bee brains. These include the honeybee genes homologous to Dank2, coracle, pumilio, and human otoferlin. Dank2 is one of two Drosophila ankyrin genes (Bouley et al., 2000). The ankyrins are linker proteins, which connect various membrane proteins with submembranous actin-spectrin skeleton. The increase of the ankyrin mRNA level may indicate that the total number of membrane protein interactions with underlying cytoskeleton is increased in the old forager brains. coracle (cora) encodes a Drosophila Protein 4.1 homolog (Lamb et al., 1998). The analysis of Drosophila cora mutants revealed that Cora is a necessary structural component of a septate junction required for the maintenance of trans-epithelial barrier and also involved in cell signaling events. pumilio (pum) encodes an RNA binding protein regulating mRNA translation. In Drosophila, pum is expressed in a subset of CNS cells and necessary for pathfinding of Bolwig nerve and motor neurons (Schmucker et al., 1997). Thus, the honeybee Pum homolog would be expressed at higher level in the specific cells of the old forager brain and may function for the correct targeting of preexisting neurons during the neuronal reorganization. Human otoferlin was identified as the gene underlying an autosomal recessive, nonsyndromic prelingual deafness, DFNB9. It is a member of a mammalian gene family related to Caenorhabditis elegans fer-1 and suggested to be involved in vesicle membrane fusion (Yasunaga et al., 1999). The upregulation of honeybee otoferlin in the old forager brains may demonstrate that the synaptic vesicle fusion is more activated.

The honeybee genes homologous to Drosophila CG2611, 4702, 7781, 7886, 9259, 9487, and 9497 are expressed at higher levels in the old forager brains. The functions of these gene products remain to be established.

The expression of several genes identified by macroarray analysis was also analyzed by Northern blot hybridization. PolyA+RNA was isolated from the brains of the bees at 0, 7, and 28 days after eclosion. The 7 days old bees perform the in-hive tasks and 28 days old bees are the pollen foragers. The relative levels of betaine/GABA transporter, Na+/K+-transporting ATPase α subunit, PI-PLC epsilon, orphan RTK, and Drosophila GH11322 homolog mRNAs in three different bee populations are shown in Fig. 1. In these experiments, the honeybee EF-1 α mRNA was used as a control to normalize the level of loaded RNA. The expression of the betaine/GABA transporter, Na+/K+-transporting ATPase α subunit, PI-PLC epsilon, and orphan RTK genes increases in the 7 and 28 days old bees relative to the 0 day old bees. There is a marked increase of betaine/GABA transporter and orphan RTK mRNAs in 28 days old bees. Meanwhile, Drosophila GH11322 homolog mRNA is already decreased in 7 days old in-hive bees and remains constant in 28 days old pollen foragers. These results are consistent with the data obtained by the macroarray analysis. In summary, the gene expression patterns of the newly emerged bee brains and the old forager brains are quite different as described. The further experiments will be necessary to address whether the changes of the gene expression patterns in honeybee brains are causally related to the age or behavioral difference.


This study was supported by Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science to YK and TK.



E. Akutagawa and M. Konishi . 2001. A monoclonal antibody specific to a song system nuclear antigen in estrildine finches. Neuron 31:545–556. Google Scholar


S. F. Altschul, T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman . 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402. Google Scholar


Y. Ben-Shahar, A. Robichon, M. B. Sokolowski, and G. E. Robinson . 2002. Influence of gene action across different time scales on behavior. Science 296:741–744. Google Scholar


M. T. Besson, L. Soustelle, and S. Birman . 1999. Identification and structural characterization of two genes encoding glutamate transporter homologues differently expressed in the nervous system of Drosophila melanogaster. FEBS lett 443:97–104. Google Scholar


K. D. Bodily, C. M. Morrison, R. B. Renden, and K. Broadie . 2001. A novel member of the Ig superfamily, turtle, is a CNS-specific protein required for coordinated motor control. J Neurosci 21:3113–3125. Google Scholar


L. A. Borden, K. E. Smith, E. L. Gustafson, T. A. Branchek, and R. L. Weinshank . 1995. Cloning and expression of a betaine/GABA transporter from human brain. J Neurochem 64:977–984. Google Scholar


M. Boube, C. Faucher, L. Joulia, D. L. Cribbs, and H-M. Bourbon . 2000. Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification. Genes Dev 14:2906–2917. Google Scholar


M. Bouley, M. Z. Tian, K. Paisley, Y. C. Shen, J. D. Malhotra, and M. Hortsch . 2000. The L1-type cell adhesion molecule neuroglian influences the stability of neural ankyrin in the Drosophila embryo but not its axonal localization. J Neurosci 20:4515–4523. Google Scholar


S. E. Fahrbach and G. E. Robinson . 1995. Behavioral development in the honey bee: Toward the study of learning under natural conditions. Learn Mem 2:199–224. Google Scholar


S. E. Fahrbach and G. E. Robinson . 1996. Juvenile hormone, behavioral maturation, and brain structure in the honey bee. Dev Neurosci 18:102–114. Google Scholar


S. M. Farris, G. E. Robinson, and S. E. Fahrbach . 2001. Experience-and agerelated outgrowth of intrinsic neurons in the mushroom bodies of the adult worker honeybee. J Neurosci 21:6395–6404. Google Scholar


M. Gahr and R. Metzdorf . 1997. Distribution and dynamics in the expression of androgen and estrogen receptors in vocal control systems of songbirds. Brain Res Bull 44:509–517. Google Scholar


N. Harden, S. F. Yap, M. A. Chiam, and L. Lim . 1993. A Drosophila gene encoding a protein with similarity to diacylglycerol kinase is expressed in specific neurons. Biochem J 289:439–444. Google Scholar


R. Hoshide, Y. Ikeda, S. Karashima, T. Matsuura, S. Komaki, T. Kishino, N. Niikawa, F. Endo, and I. Matsuda . 1996. Molecular cloning, tissue distribution, and chromosomal localization of human cationic amino aid transporter 2 (HCAT2). Genomics 38:174–178. Google Scholar


N. Klugbauer, L. Lacinova, E. Marais, M. Hobom, and F. Hofmann . 1999. Molecular diversity of the calcium channel α2 δ subunit. J Neurosci 19:684–691. Google Scholar


N. X. Krueger, M. Streuli, and H. Saito . 1990. Structural diversity and evolution of human receptor-like protein tyrosine phosphatases. EMBO J 9:3241–3252. Google Scholar


R. S. Lamb, R. E. Ward, L. Schweizer, and R. G. Fehon . 1998. Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in embryonic and adult epithelial cells. Mol Biol Cell 9:3505–3519. Google Scholar


R. Menzel and U. Muller . 1996. Learning and memory in honeybees: From behavior to neural substrates. Annu Rev Neurosci 19:379–404. Google Scholar


R. Menzel and M. Giurfa . 2001. Cognitive architecture of a mini-brain: the honeybee. Trends Cogn Sci 5:62–71. Google Scholar


T. P. Neufeld, A. H. Tang, and G. M. Rubin . 1998. A genetic screen to identify components of the sina signaling pathway in Drosophila eye development. Genetics 148:277–286. Google Scholar


D. N. T. Nguyen, Y. Liu, M. L. Litsky, and R. Reinke . 1997. The sidekick gene, a member of the immunoglobulin superfamily, is required for pattern formation in the Drosophila eye. Development 124:3303–3312. Google Scholar


I. Oishi, S. Takeuchi, R. Hashimoto, A. Nagabukuro, T. Ueda, Z-J. Liu, T. Hatta, S. Akira, Y. Matsuda, H. Yamamura, H. Otani, and Y. Minami . 1999. Spatio-temporally regulated expression of receptor tyrosine kinases, mRor1, mRor2, during mouse development: implications in development and function of the nervous system. Genes Cells 4:41–56. Google Scholar


T. Okuda, T. Haga, Y. Kanai, H. Endou, T. Ishihara, and I. Katsura . 2000. Identification and characterization of the high-affinity choline transporter. Nat Neurosci 3:120–125. Google Scholar


T. Olsson, A. H. Mohammed, L. F. Donaldson, B. G. Henriksson, and J. R. Seckl . 1994. Glucocorticoid receptor and NGFI-A gene expression are induced in the hippocampus after environmental enrichment in adult rats. Brain Res Mol Brain Res 23:349–353. Google Scholar


S. Rasmuson, T. Olsson, B. G. Henriksson, P. A. Kelly, M. C. Holmes, J. R. Seckl, and A. H. Mohammed . 1998. Environmental enrichment selectively increases 5-HT1A receptor mRNA expression and binding in the rat hippocampus. Brain Res Mol Brain Res 53:285–290. Google Scholar


M. F. Romero, D. Henry, S. Nelson, P. J. Harte, A. K. Dillon, and C. M. Sciortino . 2000. Cloning and characterization of a Na+-driven anion exchanger (NDAE1). J Biol Chem 275:24552–24559. Google Scholar


D. Schmucker, H. Jackle, and U. Gaul . 1997. Genetic analysis of the larval optic nerve projection in Drosophila. Development 124:937–948. Google Scholar


S. Shah and D. R. Hyde . 1995. Two Drosophila genes that encode the alpha and beta subunits of the brain soluble guanylyl cyclase. J Biol Chem 270:15368–15376. Google Scholar


D. Sigg, C. M. Thompson, and A. R. Mercer . 1997. Activity-dependent changes to the brain and behavior of the honey bee, Apis mellifera (L.). J Neurosci 17:7148–7156. Google Scholar


M. Sone, E. Suzuki, M. Hoshino, D. Hou, H. Kuromi, M. Fukata, S. Kuroda, K. Kaibuchi, Y. Nabeshima, and C. Hama . 2000. Synaptic development is controlled in the periactive zones of Drosophila synapses. Development 127:4157–4168. Google Scholar


C. Song, C-D. Hu, M. Masago, K. Kariya, Y. Yamawaki-Kataoka, M. Shibatohge, D. Wu, T. Satoh, and T. Kataoka . 2001. Regulation of a novel human phospholipase C, PLC, through membrane targeting by Ras. J Biol Chem 276:2752–2757. Google Scholar


B. Sun, W. Wang, and P. M. Salvaterra . 1998. Functional analysis and tissue-specific expression of Drosophila Na+, K+-ATPase subunits. J Neurochem 71:142–151. Google Scholar


D. P. Toma, G. Bloch, D. Moore, and G. E. Robinson . 2000. Changes in period mRNA levels in the brain and division of labor in honey bee colonies. Proc Natl Acad Sci USA 97:6914–6919. Google Scholar


C. Wagener-Hulme, J. C. Kuehn, D. J. Schulz, and G. E. Robinson . 1999. Biogenic amines and division of labor in honey bee colonies. J Comp Physiol 184:471–479. Google Scholar


G. S. Withers, S. E. Fahrbach, and G. E. Robinson . 1993. Selective neuroanatomical plasticity and division of labour in the honeybee. Nature 364:238–240. Google Scholar


S. Yasunaga, M. Grati, M. Cohen-Salmon, A. El-Amraoui, M. Mustapha, N. Salem, E. El-Zir, J. Loiselet, and C. Petit . 1999. A mutation in OTOF, encoding otoferlin, a FER-1-like protein, causes DFNB9, a nonsyndromic form of deafness. Nat Genet 21:363–369. Google Scholar
Mayumi Tsuchimoto, Makoto Aoki, Mamoru Takada, Yoshinori Kanou, Hiromi Sasagawa, Yasuo Kitagawa, and Tatsuhiko Kadowaki "The Changes of Gene Expression in Honeybee (Apis mellifera) Brains Associated with Ages," Zoological Science 21(1), 23-28, (27 January 2004).[23:TCOGEI]2.0.CO;2
Received: 2 May 2003; Accepted: 1 June 2003; Published: 27 January 2004
gene expression
Back to Top