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1 January 2005 Prolactin, an Activator of Epithelial Na Channel, Inhibits Basolateral K Channels in Adult Tree Frog Skin
Makoto Takada
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Abstract

Adult amphibian skin actively transports Na from its apical to basolateral side while in turn, K is recycled through Na , K -ATPase and K channels located in the basolateral membrane. We previously found that PRL stimulates Na transport in the skin of the adult tree frog (Hyla arborea japonica) via an increase in the open-channel density of the epithelial Na channel (ENaC). If PRL also activates basolateral K channels, this activation would help to stimulate Na transport, too. Whether PRL does indeed stimulate basolateral K channels in the adult tree frog was examined by measuring the short-circuit current across nystatin-treated skin. Both tolbutamide, a KATP channel blocker, and tetrapentylammonium (TPA), a KCa channel blocker, blocked the current, the effect of TPA being more powerful than that of tolbutamide. Contrary to expectation, PRL inhibited the basolateral K channels in this skin. In the presence of basolateral amiloride, PRL still inhibited the basolateral K current, suggesting that the Na -H exchanger located in the basolateral membrane does not mediate the inhibitory effect of PRL on the basolateral K channels in Hyla.

Makoto Takada "Prolactin, an Activator of Epithelial Na Channel, Inhibits Basolateral K Channels in Adult Tree Frog Skin," Zoological Science 22(1), 95-100, (1 January 2005). https://doi.org/10.2108/zsj.22.95
Received: 26 May 2004; Accepted: 1 October 2004; Published: 1 January 2005
KEYWORDS
basolateral K channel
epithelial transport
frog skin
prolactin
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