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1 August 2005 Presence of a Cytoplasmic Retention Sequence within the Human Interleukin-1α Precursor
Makoto Sudo, Yoshiro Kobayashi, Naoko Watanabe
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Interleukin (IL)-1α is primarily translated as a 33 kDa molecule (IL-1α1–271), and then processed into a 17 kDa molecule (IL-1α119–271) by calpain. The precursor region of IL-1α (IL-1α1–118) contains a nuclear localization signal (KVLKKRRL, residues 79–86). We investigated the intracellular localization IL-1α fused with green fluorescent protein or β-galactosidase. IL-1α1–118 was localized exclusively in nucleus, but IL-1α1–271 in both the nucleus and the cytoplasm, suggesting the presence of a cytoplasmic retention signal within the mature region of IL-1α. Furthermore, the intracellular localization of IL-1α with deletions from the C terminus, internal deletions and point mutations suggested that the cytoplasmic retention signal is located within residues 168–201.

Makoto Sudo, Yoshiro Kobayashi, and Naoko Watanabe "Presence of a Cytoplasmic Retention Sequence within the Human Interleukin-1α Precursor," Zoological Science 22(8), 891-896, (1 August 2005).
Received: 11 May 2005; Accepted: 1 June 2005; Published: 1 August 2005
cytoplasmic retention signal
green fluorescent protein
interleukin-1α precursor
nuclear localization signal
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