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1 October 2006 Adaptive Changes in TEF-1 Gene Expression During Cold Acclimation in the Medaka
Yukihiko Yamasaki, Yuta Komoike, Toru Higashinakagawa
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How animals adaptively respond to a cold or hot environment has been questioned for a long time. Recently, with the aid of microarray analysis, various temperature-sensitive genes have been identified in several species. However, a definitive hypothesis regarding the mechanism of adaptation has not been proposed. In the present study, we surveyed, in medaka (Oryzias latipes), genes for which the level of expression changes depending on the surrounding temperature. A messenger RNA differential display of medaka muscle total RNA revealed one such gene encoding transcription enhancer factor-1 (TEF-1). In medaka muscle, the TEF-1 gene produces two splicing variants, TEF-1A and TEF-1B mRNAs. During cold acclimation, the mRNA level of TEF-1A decreased, whereas that of TEF-1B increased. We also found that three putative downstream genes of TEF-1, two for myosin heavy chain (MyHC) and one for troponin T (TnT), a specific group of muscle proteins, were transcribed in a temperature-dependent manner. These results suggest that the transcription of MyHC and/or TnT is regulated by TEF-1 and that these molecules participate in muscle reconstruction during temperature adaptation in fish.

Yukihiko Yamasaki, Yuta Komoike, and Toru Higashinakagawa "Adaptive Changes in TEF-1 Gene Expression During Cold Acclimation in the Medaka," Zoological Science 23(10), 903-908, (1 October 2006).
Received: 2 March 2006; Accepted: 1 June 2006; Published: 1 October 2006
mRNA differential display
splicing variant
temperature acclimation
transcription enhancer factor-1
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