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1 August 2006 Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis
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Abstract

We previously identified a 66 kDa axonemal protein (Ci-Axp66.0) in sperm of the ascidian Ciona intestinalis, Here we found that Ci-Axp66.0 shows sequence similarity to the DC2 subunit of the Chlamydomonas outer arm docking complex. Analysis of secondary structure of Ci-Axp66.0 suggested that the N-terminal two-thirds of the molecule is rich in coiled coil structure, as in Chlamydomonas DC2. Immunogold localization revealed that it is located in the vicinity of outer arm dynein. Ci-Axp66.0 was partly extracted from the axonemes by a high salt solution and co-purified with outer arm dynein. This co-purification was not affected by the absence of Mg2 in isolation buffer, indicating that Ci-Axp66.0 is associated with outer arm dynein. These results suggest that Ci-Axp66.0 is a component of the outer arm dynein docking complex in the axonemes of Ciona sperm.

Yuji Ushimaru, Aru Konno, Maiko Kaizu, Kazuo Ogawa, Nori Satoh, and Kazuo Inaba "Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis," Zoological Science 23(8), 679-687, (1 August 2006). https://doi.org/10.2108/zsj.23.679
Received: 14 September 2005; Accepted: 1 January 2006; Published: 1 August 2006
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