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1 December 2008 Isolation of Novel Isoforms of Estrogen Receptor Genes from Xenopus Gonad and Brain
Junshin Iwabuchi, Koji Arai, Shohei Miyata
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Abstract

We isolated four variants of ER α mRNA, two each from the brain and gonad of the Xenopus embryo (brain, bER α 1 and bER α 2; gonad, gER α 1 and gER α 2). The N-terminal-domain of ER α differed between the brain and gonad. While the C-terminal region downstream of the DNA-binding domain of ER α differed between ER α 1 and ER α 2 in both the brain and gonad, each of ER α 1 and ER α 2 contained the same sequence sets (bER α 1 and gER α 1; bER α 2 and gER α 2) between the brain and gonad. Both bER α 1 and gER α 1 contained the same full-length, C-terminal estrogen receptor, whereas the C terminus of bER α 2 and gER α 2 lacked half of the DNA-binding, ligand-binding, and transcriptional activation domain. Although gER α was mainly expressed in the gonad, both bER α and gER α were expressed in the brain of the Xenopus embryo at stage 50. These isoforms might be transcribed by alterative splicing of a single gene. Two gonad ER β s differing in the length of the N-terminal sequence were isolated from the Xenopus gonad. While ER β was expressed in the gonad, it was not expressed in the brain of the Xenopus embryo at stage 50.

Junshin Iwabuchi, Koji Arai, and Shohei Miyata "Isolation of Novel Isoforms of Estrogen Receptor Genes from Xenopus Gonad and Brain," Zoological Science 25(12), 1227-1233, (1 December 2008). https://doi.org/10.2108/zsj.25.1227
Received: 7 June 2008; Accepted: 1 September 2008; Published: 1 December 2008
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