Translator Disclaimer
25 July 2008 Detection and Changes in Levels of Testosterone During Spermatogenesis in the Freshwater Planarian Bdellocephala brunnea
Author Affiliations +
Abstract

It was reported recently that vertebrate-type steroids exist and control reproduction in several groups of invertebrates, including molluscs. Sexually reproductive freshwater planarians of the species Bdellocephala brunnea have a limited breeding season in their natural habitat. This phenomenon suggests that some endogenous reproductive hormones might play a role in vivo. However, to date, sex steroids such as androgen, estrogen, and progesterone have not been found in planarians. The goal of the present study was to determine whether androgen is present in sexual planarians such as B. brunnea. The presence of testosterone was detected by high-pressure liquid chromatography and, in sexually reproductive individuals in which no seminal vesicles were visible, the level of testosterone was about twice than that in individuals with visible seminal vesicles. An enzyme-linked immunosorbent assay revealed that the levels of testosterone during terminal spermatogenesis were three times higher than during the spermatocyte-building phase. Our results indicate that sexually reproductive freshwater planarians such as B. brunnea might have vertebrate-type steroids and show variation in testosterone levels during spermatogenesis.

Makoto Fukushima, Ikumi Funabiki, Tsutomu Hashizume, Kyoichi Osada, Wataru Yoshida, and Sachiko Ishida "Detection and Changes in Levels of Testosterone During Spermatogenesis in the Freshwater Planarian Bdellocephala brunnea," Zoological Science 25(7), 760-765, (25 July 2008). https://doi.org/10.2108/zsj.25.760
Received: 3 December 2007; Accepted: 1 April 2008; Published: 25 July 2008
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top